Leginon: A system for fully automated acquisition of 1000 electron micrographs a day

C. S. Potter, H. Chu, B. Frey, C. Green, N. Kisseberth, T. J. Madden, K. L. Miller, K. Nahrstedt, J. Pulokas, A. Reilein, D. Tcheng, D. Weber, B. Carragher

Research output: Contribution to journalArticle

Abstract

We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs.

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalUltramicroscopy
Volume77
Issue number3-4
DOIs
StatePublished - Jul 1999

Keywords

  • Automated data acquisition
  • Instrument control
  • Microscopic methods
  • Three- dimensional electron microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Instrumentation

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    Potter, C. S., Chu, H., Frey, B., Green, C., Kisseberth, N., Madden, T. J., Miller, K. L., Nahrstedt, K., Pulokas, J., Reilein, A., Tcheng, D., Weber, D., & Carragher, B. (1999). Leginon: A system for fully automated acquisition of 1000 electron micrographs a day. Ultramicroscopy, 77(3-4), 153-161. https://doi.org/10.1016/S0304-3991(99)00043-1