Leaf photosynthesis and carbohydrate dynamics of soybeans grown throughout their life-cycle under Free-Air Carbon dioxide Enrichment

A. Rogers, D. J. Allen, P. A. Davey, P. B. Morgan, E. A. Ainsworth, C. J. Bernacchi, G. Cornic, O. Dermody, F. G. Dohleman, E. A. Heaton, J. Mahoney, X. G. Zhu, E. H. Delucia, D. R. Ort, S. P. Long

Research output: Contribution to journalArticle

Abstract

A lower than theoretically expected increase in leaf photosynthesis with long-term elevation of carbon dioxide concentration ([CO2]) is often attributed to limitations in the capacity of the plant to utilize the additional photosynthate, possibly resulting from restrictions in rooting volume, nitrogen supply or genetic constraints. Field-grown, nitrogen-fixing soybean with indeterminate flowering might therefore be expected to escape these limitations. Soybean was grown from emergence to grain maturity in ambient air (372 μmol mol-1 [CO2]) and in air enriched with CO2 (552 μmol mol-1 [CO2]) using Free-Air CO2 Enrichment (FACE) technology. The diurnal courses of leaf CO2 uptake (A) and stomatal conductance (gs) for upper canopy leaves were followed throughout development from the appearance of the first true leaf to the completion of seed filling. Across the growing season the daily integrals of leaf photosynthetic CO2 uptake (A′) increased by 24.6% in elevated [CO2] and the average mid-day gs decreased by 21.9%. The increase in A′ was about half the 44.5% theoretical maximum increase calculated from Rubisco kinetics. There was no evidence that the stimulation of A was affected by time of day, as expected if elevated [CO2] led to a large accumulation of leaf carbohydrates towards the end of the photoperiod. In general, the proportion of assimilated carbon that accumulated in the leaf as non-structural carbohydrate over the photoperiod was small (< 10%) and independent of [CO2] treatment. By contrast to A′, daily integrals of PSII electron transport measured by modulated chlorophyll fluorescence were not significantly increased by elevated [CO2]. This indicates that A at elevated [CO 2] in these field conditions was predominantly ribulose-1,5- bisphosphate (RubP) limited rather than Rubisco limited. There was no evidence of any loss of stimulation toward the end of the growing season; the largest stimulation of A′ occurred during late seed filling. The stimulation of photosynthesis was, however, transiently lost for a brief period just before seed fill. At this point, daytime accumulation of foliar carbohydrates was maximal, and the hexose:sucrose ratio in plants grown at elevated [CO 2] was significantly larger than that in plants grown at current [CO2]. The results show that even for a crop lacking the constraints that have been considered to limit the responses of C3 plants to rising [CO2] in the long term, the actual increase in A over the growing season is considerably less than the increase predicted from theory.

Original languageEnglish (US)
Pages (from-to)449-458
Number of pages10
JournalPlant, Cell and Environment
Volume27
Issue number4
DOIs
StatePublished - Apr 1 2004

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free air carbon dioxide enrichment
Photosynthesis
Life Cycle Stages
Soybeans
Carbon Dioxide
life cycle (organisms)
carbon dioxide
Air
Carbohydrates
photosynthesis
soybeans
Ribulose-Bisphosphate Carboxylase
carbohydrates
Seeds
Photoperiod
Carbon Monoxide
leaves
Nitrogen
Hexoses
Chlorophyll

Keywords

  • Atmospheric change
  • Chlorophyll fluorescence
  • Electron transport
  • Elevated carbon dioxide concentration
  • FACE
  • Global climate change
  • Glycine max
  • Photosynthesis
  • Stomatal conductance

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Leaf photosynthesis and carbohydrate dynamics of soybeans grown throughout their life-cycle under Free-Air Carbon dioxide Enrichment. / Rogers, A.; Allen, D. J.; Davey, P. A.; Morgan, P. B.; Ainsworth, E. A.; Bernacchi, C. J.; Cornic, G.; Dermody, O.; Dohleman, F. G.; Heaton, E. A.; Mahoney, J.; Zhu, X. G.; Delucia, E. H.; Ort, D. R.; Long, S. P.

In: Plant, Cell and Environment, Vol. 27, No. 4, 01.04.2004, p. 449-458.

Research output: Contribution to journalArticle

Rogers, A. ; Allen, D. J. ; Davey, P. A. ; Morgan, P. B. ; Ainsworth, E. A. ; Bernacchi, C. J. ; Cornic, G. ; Dermody, O. ; Dohleman, F. G. ; Heaton, E. A. ; Mahoney, J. ; Zhu, X. G. ; Delucia, E. H. ; Ort, D. R. ; Long, S. P. / Leaf photosynthesis and carbohydrate dynamics of soybeans grown throughout their life-cycle under Free-Air Carbon dioxide Enrichment. In: Plant, Cell and Environment. 2004 ; Vol. 27, No. 4. pp. 449-458.
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AU - Rogers, A.

AU - Allen, D. J.

AU - Davey, P. A.

AU - Morgan, P. B.

AU - Ainsworth, E. A.

AU - Bernacchi, C. J.

AU - Cornic, G.

AU - Dermody, O.

AU - Dohleman, F. G.

AU - Heaton, E. A.

AU - Mahoney, J.

AU - Zhu, X. G.

AU - Delucia, E. H.

AU - Ort, D. R.

AU - Long, S. P.

PY - 2004/4/1

Y1 - 2004/4/1

N2 - A lower than theoretically expected increase in leaf photosynthesis with long-term elevation of carbon dioxide concentration ([CO2]) is often attributed to limitations in the capacity of the plant to utilize the additional photosynthate, possibly resulting from restrictions in rooting volume, nitrogen supply or genetic constraints. Field-grown, nitrogen-fixing soybean with indeterminate flowering might therefore be expected to escape these limitations. Soybean was grown from emergence to grain maturity in ambient air (372 μmol mol-1 [CO2]) and in air enriched with CO2 (552 μmol mol-1 [CO2]) using Free-Air CO2 Enrichment (FACE) technology. The diurnal courses of leaf CO2 uptake (A) and stomatal conductance (gs) for upper canopy leaves were followed throughout development from the appearance of the first true leaf to the completion of seed filling. Across the growing season the daily integrals of leaf photosynthetic CO2 uptake (A′) increased by 24.6% in elevated [CO2] and the average mid-day gs decreased by 21.9%. The increase in A′ was about half the 44.5% theoretical maximum increase calculated from Rubisco kinetics. There was no evidence that the stimulation of A was affected by time of day, as expected if elevated [CO2] led to a large accumulation of leaf carbohydrates towards the end of the photoperiod. In general, the proportion of assimilated carbon that accumulated in the leaf as non-structural carbohydrate over the photoperiod was small (< 10%) and independent of [CO2] treatment. By contrast to A′, daily integrals of PSII electron transport measured by modulated chlorophyll fluorescence were not significantly increased by elevated [CO2]. This indicates that A at elevated [CO 2] in these field conditions was predominantly ribulose-1,5- bisphosphate (RubP) limited rather than Rubisco limited. There was no evidence of any loss of stimulation toward the end of the growing season; the largest stimulation of A′ occurred during late seed filling. The stimulation of photosynthesis was, however, transiently lost for a brief period just before seed fill. At this point, daytime accumulation of foliar carbohydrates was maximal, and the hexose:sucrose ratio in plants grown at elevated [CO 2] was significantly larger than that in plants grown at current [CO2]. The results show that even for a crop lacking the constraints that have been considered to limit the responses of C3 plants to rising [CO2] in the long term, the actual increase in A over the growing season is considerably less than the increase predicted from theory.

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KW - Atmospheric change

KW - Chlorophyll fluorescence

KW - Electron transport

KW - Elevated carbon dioxide concentration

KW - FACE

KW - Global climate change

KW - Glycine max

KW - Photosynthesis

KW - Stomatal conductance

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