Lasing at 602-620 nm from a red algae-derived phycobiliprotein

José A. Rivera; J. Gary Eden

doi:10.1063/1.4999716

Lasing at 602-620 nm from a red algae-derived phycobiliprotein

José A. Rivera, J. Gary Eden

Electrical and Computer Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Lasing in the 602-620 nm (orange-red) spectral region has been observed from R-phycoerythrin (RPE), a phycobiliprotein responsible for the >80% quantum yield of red algae. Photoexcitation of 8 μM RPE solutions in a Fabry-Pérot resonator with frequency-doubled Nd:YAG laser pulses (λ_p = 532 nm) yields >26 nJ of energy in ∼2.5 ns (FWHM) pulses, which corresponds to >10 W of peak power. Maximum laser emission occurs at ∼610 nm, the threshold pump energy fluence is measured to be 260 ± 15 μJ/mm², and more than 100 longitudinal laser modes are generated when the cavity free spectral range and mirror separation are Δλ = 0.18 nm and L = 777 ± 1 μm, respectively. In combination with the known versatility of the phycobiliproteins as fluorescent tags, the biomolecular RPE laser reported here suggests its applicability to clinical assays and in situ laser imaging.

Original language	English (US)
Article number	121301
Journal	APL Photonics
Volume	2
Issue number	12
DOIs	https://doi.org/10.1063/1.4999716
State	Published - Dec 1 2017

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Computer Networks and Communications

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abstract = "Lasing in the 602-620 nm (orange-red) spectral region has been observed from R-phycoerythrin (RPE), a phycobiliprotein responsible for the >80% quantum yield of red algae. Photoexcitation of 8 μM RPE solutions in a Fabry-P{\'e}rot resonator with frequency-doubled Nd:YAG laser pulses (λp = 532 nm) yields >26 nJ of energy in ∼2.5 ns (FWHM) pulses, which corresponds to >10 W of peak power. Maximum laser emission occurs at ∼610 nm, the threshold pump energy fluence is measured to be 260 ± 15 μJ/mm2, and more than 100 longitudinal laser modes are generated when the cavity free spectral range and mirror separation are Δλ = 0.18 nm and L = 777 ± 1 μm, respectively. In combination with the known versatility of the phycobiliproteins as fluorescent tags, the biomolecular RPE laser reported here suggests its applicability to clinical assays and in situ laser imaging.",

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Lasing at 602-620 nm from a red algae-derived phycobiliprotein

Abstract

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