Abstract
Traditionally large-scale chromatin structure has been studied by microscopic approaches, providing direct spatial information but limited sequence context. In contrast, newer 3C (chromosome capture conformation) methods provide rich sequence context but uncertain spatial context. Recent demonstration of large, topologically linked DNA domains, hundreds to thousands of kb in size, may now link 3C data to actual chromosome physical structures, as visualized directly by microscopic methods. Yet, new data suggesting that 3C may measure cytological rather than molecular proximity prompts a renewed focus on understanding the origin of 3C interactions and dissecting the biological significance of long-range genomic interactions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 69-78 |
| Number of pages | 10 |
| Journal | Current Opinion in Cell Biology |
| Volume | 26 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 2014 |
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ASJC Scopus subject areas
- Cell Biology
Cite this
Large-scale chromatin organization : The good, the surprising, and the still perplexing. / Belmont, Andrew S.
In: Current Opinion in Cell Biology, Vol. 26, No. 1, 01.02.2014, p. 69-78.Research output: Contribution to journal › Review article
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TY - JOUR
T1 - Large-scale chromatin organization
T2 - The good, the surprising, and the still perplexing
AU - Belmont, Andrew S.
PY - 2014/2/1
Y1 - 2014/2/1
N2 - Traditionally large-scale chromatin structure has been studied by microscopic approaches, providing direct spatial information but limited sequence context. In contrast, newer 3C (chromosome capture conformation) methods provide rich sequence context but uncertain spatial context. Recent demonstration of large, topologically linked DNA domains, hundreds to thousands of kb in size, may now link 3C data to actual chromosome physical structures, as visualized directly by microscopic methods. Yet, new data suggesting that 3C may measure cytological rather than molecular proximity prompts a renewed focus on understanding the origin of 3C interactions and dissecting the biological significance of long-range genomic interactions.
AB - Traditionally large-scale chromatin structure has been studied by microscopic approaches, providing direct spatial information but limited sequence context. In contrast, newer 3C (chromosome capture conformation) methods provide rich sequence context but uncertain spatial context. Recent demonstration of large, topologically linked DNA domains, hundreds to thousands of kb in size, may now link 3C data to actual chromosome physical structures, as visualized directly by microscopic methods. Yet, new data suggesting that 3C may measure cytological rather than molecular proximity prompts a renewed focus on understanding the origin of 3C interactions and dissecting the biological significance of long-range genomic interactions.
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UR - http://www.scopus.com/inward/citedby.url?scp=84887942741&partnerID=8YFLogxK
U2 - 10.1016/j.ceb.2013.10.002
DO - 10.1016/j.ceb.2013.10.002
M3 - Review article
C2 - 24529248
AN - SCOPUS:84887942741
VL - 26
SP - 69
EP - 78
JO - Current Opinion in Cell Biology
JF - Current Opinion in Cell Biology
SN - 0955-0674
IS - 1
ER -