Large-Conductance Transmembrane Porin Made from DNA Origami

Kerstin Göpfrich, Chen Yu Li, Maria Ricci, Satya Prathyusha Bhamidimarri, Jejoong Yoo, Bertalan Gyenes, Alexander Ohmann, Mathias Winterhalter, Aleksei Aksimentiev, Ulrich F. Keyser

Research output: Contribution to journalArticlepeer-review


DNA nanotechnology allows for the creation of three-dimensional structures at nanometer scale. Here, we use DNA to build the largest synthetic pore in a lipid membrane to date, approaching the dimensions of the nuclear pore complex and increasing the pore-area and the conductance 10-fold compared to previous man-made channels. In our design, 19 cholesterol tags anchor a megadalton funnel-shaped DNA origami porin in a lipid bilayer membrane. Confocal imaging and ionic current recordings reveal spontaneous insertion of the DNA porin into the lipid membrane, creating a transmembrane pore of tens of nanosiemens conductance. All-atom molecular dynamics simulations characterize the conductance mechanism at the atomic level and independently confirm the DNA porins' large ionic conductance.

Original languageEnglish (US)
Pages (from-to)8207-8214
Number of pages8
JournalACS Nano
Issue number9
StatePublished - Sep 27 2016


  • DNA origami
  • ionic current recordings
  • lipid membrane
  • molecular dynamics
  • synthetic porin

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)


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