@article{51120af7146d44f5b1f216d6f98c9d9e,
title = "Large-Conductance Transmembrane Porin Made from DNA Origami",
abstract = "DNA nanotechnology allows for the creation of three-dimensional structures at nanometer scale. Here, we use DNA to build the largest synthetic pore in a lipid membrane to date, approaching the dimensions of the nuclear pore complex and increasing the pore-area and the conductance 10-fold compared to previous man-made channels. In our design, 19 cholesterol tags anchor a megadalton funnel-shaped DNA origami porin in a lipid bilayer membrane. Confocal imaging and ionic current recordings reveal spontaneous insertion of the DNA porin into the lipid membrane, creating a transmembrane pore of tens of nanosiemens conductance. All-atom molecular dynamics simulations characterize the conductance mechanism at the atomic level and independently confirm the DNA porins' large ionic conductance.",
keywords = "DNA origami, ionic current recordings, lipid membrane, molecular dynamics, synthetic porin",
author = "Kerstin G{\"o}pfrich and Li, {Chen Yu} and Maria Ricci and Bhamidimarri, {Satya Prathyusha} and Jejoong Yoo and Bertalan Gyenes and Alexander Ohmann and Mathias Winterhalter and Aleksei Aksimentiev and Keyser, {Ulrich F.}",
note = "Funding Information: The authors thank K. C. Godel for help with 3D sketches and E. Hemmig for critical reading of the manuscript. K.G. acknowledges funding from the Winton Programme for the Physics of Sustainability, Gates Cambridge, and the Oppenheimer Ph.D. studentship; U.F.K. from an ERC Consolidator Grant (Designerpores 647144); and M.R. from the Early Postdoc Mobility fellowship of the Swiss National Science Foundation. A.A., J.Y., and C.Y.L. acknowledge support form the National Science Foundation under grants DMR-1507985, PHY-1430124, and EEC-1227034 and the supercomputer time provided through XSEDE Allocation grant MCA05S028 and the Blue Waters petascale supercomputer system (UIUC). M.W. and S.P.B. acknowledge support from Marie Sklodowska Curie Actions within the Initial Training Networks Translocation Network, project no. 607694. Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",
year = "2016",
month = sep,
day = "27",
doi = "10.1021/acsnano.6b03759",
language = "English (US)",
volume = "10",
pages = "8207--8214",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "9",
}