Laminin-111 improves skeletal muscle stem cell quantity and function following eccentric exercise

Kai Zou, Michael de Lisio, Heather D. Huntsman, Yair Pincu, Ziad Mahmassani, Matthew Miller, Dami Olatunbosun, Tor Jensen, Marni D. Boppart

Research output: Contribution to journalArticlepeer-review


Laminin-111 (α1, β1, γ1; LM-111) is an important component of the extracellular matrix that is required for formation of skeletal muscle during embryonic development. Recent studies suggest that LM-111 supplementation can enhance satellite cell proliferation and muscle function in mouse models of muscular dystrophy. The purpose of this study was to determine the extent to which LM-111 can alter satellite and nonsatellite stem cell quantity following eccentric exercise-induced damage in young adult, healthy mice. One week following injection of LM-111 or saline, mice either remained sedentary or were subjected to a single bout of downhill running (EX). While one muscle was preserved for evaluation of satellite cell number, the other muscle was processed for isolation of mesenchymal stem cells (MSCs; Sca-1+CD45-) via FACS at 24 hours postexercise. Satellite cell number was approximately twofold higher in LM-111/EX compared with all other groups (p <.05), and the number of satellite cells expressing the proliferation marker Ki67 was 50% to threefold higher in LM-111/EX compared with all other groups (p <.05). LM-111 also increased the quantity of embryonic myosin heavy chain-positive (eMHC+) fibers in young mice after eccentric exercise (p <.05). Although MSC percentage and number were not altered, MSC proinflammatory gene expression was decreased, and hepatocyte growth factor gene expression was increased in the presence of LM-111 (p <.05). Together, these data suggest that LM-111 supplementation provides a viable solution for increasing skeletal muscle stem cell number and/or function, ultimately allowing for improvements in the regenerative response to eccentric exercise.

Original languageEnglish (US)
Pages (from-to)1013-1022
Number of pages10
JournalStem Cells Translational Medicine
Issue number9
StatePublished - 2014


  • Downhill running
  • Hepatocyte growth factor (HGF)
  • Mesenchymal stem cells
  • Repair
  • Satellite cells

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology


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