Laminar flow based microreactor for efficient regeneration of nicotinamide cofactors for biocatalysis

Seong Kee Yoon, Cheikhou Kane, Eric R. Choban, Theodore Tzedakis, Paul J A Kenis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

One of the longstanding challenges in biocatalysis is the search for methods to continuously regenerate essential cofactors such as NADH that would enable the use of a wide range of enzymes to be used in the more environmentally friendly synthesis of chiral fine chemicals including pharmaceuticals, cosmetics, and food additives. In this work, we present a microreactor-based cofactor regeneration method that exploits the mierofluidic phenomenon of laminar flow: a reactant stream and a buffer stream are introduced in a microchannel and continue to flow side by side without turbulent mixing between two electrodes that cover opposing channel walls. Adjustment of the flow rate ratio of the two streams in laminar flow enables focusing of the reactant stream closely to the cathode, thereby reversing a normally unfavorable reaction equilibrium essential for cofactor regeneration. The absence of a bulk phase in these microreactors prevents the undesired reverse reaction to take place, which has prevented the use of electrochemical cofactor regeneration in macroscale processes. Here, we demonstrate the regeneration of NADH with conversion efficiencies as high as 31%. We also show the subsequent in situ conversion of an achiral substrate, pyruvate, into a chiral product, L-lactate, within this microreactor. Moreover, our efforts to scale these microreactors into a multi-reactor system for higher throughput and an overall efficient biocatalytic process will be presented.

Original languageEnglish (US)
Title of host publication05AIChE
Subtitle of host publication2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings
Pages9082
Number of pages1
StatePublished - 2005
Externally publishedYes
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: Oct 30 2005Nov 4 2005

Other

Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
CountryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

ASJC Scopus subject areas

  • Engineering(all)

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    Yoon, S. K., Kane, C., Choban, E. R., Tzedakis, T., & Kenis, P. J. A. (2005). Laminar flow based microreactor for efficient regeneration of nicotinamide cofactors for biocatalysis. In 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings (pp. 9082)