TY - JOUR
T1 - Lacticin 481
T2 - In Vitro Reconstitution of Lantibiotic Synthetase Activity
AU - Xie, Lili
AU - Miller, Leah M.
AU - Chatterjee, Champak
AU - Averin, Olga
AU - Kelleher, Neil L.
AU - Van Der Donk, Wilfred A.
PY - 2004/1/30
Y1 - 2004/1/30
N2 - The lantibiotic lacticin 481 is synthesized on ribosomes as a prepeptide (LctA) and posttranslationally modified to its mature form. These modifications include dehydration of serines and threonines, followed by intramolecular addition of cysteines to the unsaturated amino acids, which generates cyclic thioethers. This process breaks eight chemical bonds and forms six new bonds and is catalyzed by one enzyme, LctM. We have characterized the in vitro activity of LctM, which completely processed a series of LctA mutants, displaying a permissive substrate specificity that holds promise for antibiotic engineering.
AB - The lantibiotic lacticin 481 is synthesized on ribosomes as a prepeptide (LctA) and posttranslationally modified to its mature form. These modifications include dehydration of serines and threonines, followed by intramolecular addition of cysteines to the unsaturated amino acids, which generates cyclic thioethers. This process breaks eight chemical bonds and forms six new bonds and is catalyzed by one enzyme, LctM. We have characterized the in vitro activity of LctM, which completely processed a series of LctA mutants, displaying a permissive substrate specificity that holds promise for antibiotic engineering.
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U2 - 10.1126/science.1092600
DO - 10.1126/science.1092600
M3 - Article
C2 - 14752162
AN - SCOPUS:0942268866
SN - 0036-8075
VL - 303
SP - 679
EP - 681
JO - Science
JF - Science
IS - 5658
ER -