Lacticin 481: In Vitro Reconstitution of Lantibiotic Synthetase Activity

Lili Xie, Leah M. Miller, Champak Chatterjee, Olga Averin, Neil L. Kelleher, Wilfred A. Van Der Donk

Research output: Contribution to journalArticle

Abstract

The lantibiotic lacticin 481 is synthesized on ribosomes as a prepeptide (LctA) and posttranslationally modified to its mature form. These modifications include dehydration of serines and threonines, followed by intramolecular addition of cysteines to the unsaturated amino acids, which generates cyclic thioethers. This process breaks eight chemical bonds and forms six new bonds and is catalyzed by one enzyme, LctM. We have characterized the in vitro activity of LctM, which completely processed a series of LctA mutants, displaying a permissive substrate specificity that holds promise for antibiotic engineering.

Original languageEnglish (US)
Pages (from-to)679-681
Number of pages3
JournalScience
Volume303
Issue number5658
DOIs
StatePublished - Jan 30 2004

ASJC Scopus subject areas

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    Xie, L., Miller, L. M., Chatterjee, C., Averin, O., Kelleher, N. L., & Van Der Donk, W. A. (2004). Lacticin 481: In Vitro Reconstitution of Lantibiotic Synthetase Activity. Science, 303(5658), 679-681. https://doi.org/10.1126/science.1092600