Lactic acid production from cellobiose and xylose by engineered Saccharomyces cerevisiae

Timothy L. Turner, Guo Chang Zhang, Eun Joong Oh, Vijay Subramaniam, Andrew Adiputra, Vimal Subramaniam, Christopher D. Skory, Ji Yeon Jang, Byung Jo Yu, In Park, Yong Su Jin

Research output: Contribution to journalArticlepeer-review


Efficient and rapid production of value-added chemicals from lignocellulosic biomass is an important step toward a sustainable society. Lactic acid, used for synthesizing the bioplastic polylactide, has been produced by microbial fermentation using primarily glucose. Lignocellulosic hydrolysates contain high concentrations of cellobiose and xylose. Here, we constructed a recombinant Saccharomyces cerevisiae strain capable of fermenting cellobiose and xylose into lactic acid. Specifically, genes (cdt-1, gh1-1, XYL1, XYL2, XYL3, and ldhA) coding for cellobiose transporter, β-glucosidase, xylose reductase, xylitol dehydrogenase, xylulokinase, and lactate dehydrogenase were integrated into the S. cerevisiae chromosomes. The resulting strain produced lactic acid from cellobiose or xylose with high yields. When fermenting a cellulosic sugar mixture containing 10g/L glucose, 40g/L xylose, and 80g/L cellobiose, the engineered strain produced 83g/L of lactic acid with a yield of 0.66g lactic acid/g sugar (66% theoretical maximum). This study demonstrates initial steps toward the feasibility of sustainable production of lactic acid from lignocellulosic sugars by engineered yeast. Biotechnol.

Original languageEnglish (US)
Pages (from-to)1075-1083
Number of pages9
JournalBiotechnology and bioengineering
Issue number5
StatePublished - May 1 2016


  • Cellobiose
  • Lactate dehydrogenase
  • Lactic acid
  • Metabolic engineering
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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