TY - JOUR
T1 - Label-free virus detection using silicon photonic microring resonators
AU - McClellan, Melinda S.
AU - Domier, Leslie L.
AU - Bailey, Ryan C.
N1 - Funding Information:
We acknowledge support from the NIH Director's New Innovator Award Program , part of the NIH Roadmap for Medical Research, through grant number 1-DP2-OD002190-01 . MSM is supported through a Robert C. and Carolyn J. Springborn Fellowship from the Department of Chemistry at the University of Illinois at Urbana-Champaign .
PY - 2012/1/15
Y1 - 2012/1/15
N2 - Viruses represent a continual threat to humans through a number of mechanisms, which include disease, bioterrorism, and destruction of both plant and animal food resources. Many contemporary techniques used for the detection of viruses and viral infections suffer from limitations such as the need for extensive sample preparation or the lengthy window between infection and measurable immune response, for serological methods. In order to develop a method that is fast, cost-effective, and features reduced sample preparation compared to many other virus detection methods, we report the application of silicon photonic microring resonators for the direct, label-free detection of intact viruses in both purified samples as well as in a complex, real-world analytical matrix. As a model system, we demonstrate the quantitative detection of Bean pod mottle virus, a pathogen of great agricultural importance, with a limit of detection of 10. ng/mL. By simply grinding a small amount of leaf sample in buffer with a mortar and pestle, infected leaves can be identified over a healthy control with a total analysis time of less than 45. min. Given the inherent scalability and multiplexing capability of the semiconductor-based technology, we feel that silicon photonic microring resonators are well-positioned as a promising analytical tool for a number of viral detection applications.
AB - Viruses represent a continual threat to humans through a number of mechanisms, which include disease, bioterrorism, and destruction of both plant and animal food resources. Many contemporary techniques used for the detection of viruses and viral infections suffer from limitations such as the need for extensive sample preparation or the lengthy window between infection and measurable immune response, for serological methods. In order to develop a method that is fast, cost-effective, and features reduced sample preparation compared to many other virus detection methods, we report the application of silicon photonic microring resonators for the direct, label-free detection of intact viruses in both purified samples as well as in a complex, real-world analytical matrix. As a model system, we demonstrate the quantitative detection of Bean pod mottle virus, a pathogen of great agricultural importance, with a limit of detection of 10. ng/mL. By simply grinding a small amount of leaf sample in buffer with a mortar and pestle, infected leaves can be identified over a healthy control with a total analysis time of less than 45. min. Given the inherent scalability and multiplexing capability of the semiconductor-based technology, we feel that silicon photonic microring resonators are well-positioned as a promising analytical tool for a number of viral detection applications.
KW - Bean pod mottle virus
KW - Bioterrorism
KW - Label-free biosensor
KW - Microring resonator
KW - Silicon photonics
KW - Virus detection
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U2 - 10.1016/j.bios.2011.10.056
DO - 10.1016/j.bios.2011.10.056
M3 - Article
C2 - 22138465
AN - SCOPUS:84455205520
SN - 0956-5663
VL - 31
SP - 388
EP - 392
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 1
ER -