Label-free tomography of strongly scattering specimens

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Many specimens of interest, embryos, brain slices, organoids, are thick and optically inhomogeneous, which results in strong multiple scattering and low-contrast imaging. We developed GLIM as a novel label-free imaging method with applicability from nanoscale topographic structures to 2-300-micron thick tissues. Due to its interferometric principle that maintains equal power in the two interfering fields, GLIM is able to reject much of the multiple scattering background and generate high contrast interference. We present its principle and illustrate the performance with imaging nano pillars, cells, and embryos.

Original languageEnglish (US)
Title of host publicationDigital Holography and Three-Dimensional Imaging, DH 2017
PublisherOSA - The Optical Society
ISBN (Electronic)9781557528209
DOIs
StatePublished - Jan 1 2017
EventDigital Holography and Three-Dimensional Imaging, DH 2017 - JeJu Island, Korea, Republic of
Duration: May 29 2017Jun 1 2017

Publication series

NameOptics InfoBase Conference Papers
VolumePart F47-DH 2017

Other

OtherDigital Holography and Three-Dimensional Imaging, DH 2017
CountryKorea, Republic of
CityJeJu Island
Period5/29/176/1/17

Fingerprint

Tomography
Labels
Multiple scattering
Scattering
Imaging techniques
Brain
Tissue

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials

Cite this

Popescu, G. (2017). Label-free tomography of strongly scattering specimens. In Digital Holography and Three-Dimensional Imaging, DH 2017 (Optics InfoBase Conference Papers; Vol. Part F47-DH 2017). OSA - The Optical Society. https://doi.org/10.1364/DH.2017.Tu2A.1

Label-free tomography of strongly scattering specimens. / Popescu, Gabriel.

Digital Holography and Three-Dimensional Imaging, DH 2017. OSA - The Optical Society, 2017. (Optics InfoBase Conference Papers; Vol. Part F47-DH 2017).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Popescu, G 2017, Label-free tomography of strongly scattering specimens. in Digital Holography and Three-Dimensional Imaging, DH 2017. Optics InfoBase Conference Papers, vol. Part F47-DH 2017, OSA - The Optical Society, Digital Holography and Three-Dimensional Imaging, DH 2017, JeJu Island, Korea, Republic of, 5/29/17. https://doi.org/10.1364/DH.2017.Tu2A.1
Popescu G. Label-free tomography of strongly scattering specimens. In Digital Holography and Three-Dimensional Imaging, DH 2017. OSA - The Optical Society. 2017. (Optics InfoBase Conference Papers). https://doi.org/10.1364/DH.2017.Tu2A.1
Popescu, Gabriel. / Label-free tomography of strongly scattering specimens. Digital Holography and Three-Dimensional Imaging, DH 2017. OSA - The Optical Society, 2017. (Optics InfoBase Conference Papers).
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