Label-free tomography of strongly scattering specimens

Gabriel Popescu

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Many specimens of interest, embryos, brain slices, organoids, are thick and optically inhomogeneous, which results in strong multiple scattering and low-contrast imaging. We developed GLIM as a novel label-free imaging method with applicability from nanoscale topographic structures to 2-300-micron thick tissues. Due to its interferometric principle that maintains equal power in the two interfering fields, GLIM is able to reject much of the multiple scattering background and generate high contrast interference. We present its principle and illustrate the performance with imaging nano pillars, cells, and embryos.

Original languageEnglish (US)
Title of host publicationDigital Holography and Three-Dimensional Imaging, DH 2017
PublisherOptica Publishing Group (formerly OSA)
ISBN (Print)9781943580286
StatePublished - 2017
EventDigital Holography and Three-Dimensional Imaging, DH 2017 - JeJu Island, Korea, Republic of
Duration: May 29 2017Jun 1 2017

Publication series

NameOptics InfoBase Conference Papers
VolumePart F47-DH 2017
ISSN (Electronic)2162-2701


OtherDigital Holography and Three-Dimensional Imaging, DH 2017
Country/TerritoryKorea, Republic of
CityJeJu Island

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials


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