Label-free photonic crystal biosensor integrated microfluidic chip for determination of kinetic reaction rate constants

Charles J. Choi, Ian D. Block, Brian Bole, David Dralle, Brian T. Cunningham

Research output: Contribution to journalArticlepeer-review

Abstract

We demonstrate a photonic crystal integrated microfluidic chip that is compatible with a 384-well microplate format for measuring kinetic reaction rate constants in high-throughput biomolecular interaction screening applications. The device enables low volume kinetic analysis of proteinprotein interactions with low flow latency, and control of five analyte flow channels with a single control point. The structure is fabricated with a replica molding process that produces the submicron photonic crystal structure simultaneously with the micrometer-scale fluid channel structure. The device significantly reduces the kinetic assay time required compared with a conventional biosensor microplate in which reagents reach the active detection surface by diffusion. Using the photonic crystal sensor fluid network system, we demonstrate determination of the kinetic association/dissociation rate constants between immobilized ligands and analytes in the flow stream, using the heparin/lactoferrin system as an example.

Original languageEnglish (US)
Article number5290394
Pages (from-to)1697-1704
Number of pages8
JournalIEEE Sensors Journal
Volume9
Issue number12
DOIs
StatePublished - Dec 2009

Keywords

  • Biomedical transducers
  • Flexible structures
  • Optical resonance

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Instrumentation

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