Abstract

The ability to study interactions among biomolecules, to observe the activity of cells, and to detect analytes specifically from bodily fluids, manufacturing processes, or environmental samples are cornerstones of life science research, pharmaceutical discovery, medical diagnosis, and food/water safety assurance. These capabilities and many others are enabled by the ability to perform biochemical and cell-based assays that allow scientists to ask basic questions about whether one analyte interacts with another, how strong the binding affinity is between two proteins, whether a chemical compound will affect the proliferation rate of cancer cells, and the concentration of a biomarker for cancer within a patient's blood sample. The development of technology to meet these requirements is challenging because biochemical analytes, which can include drug compounds with molecular weights below 500 Da, DNA oligomers, peptides, enzymes, anti-bodies, and viral particles, are exceedingly small and sometimes present within a test sample at concentrations in the fg/ml to pg/ml concentration range that simultaneously contains thousands of other molecules at concentrations orders of magnitude greater. Larger biochemical analytes – such as bacteria, spores, cells, and cell clusters – are less difficult to observe if they can be stained with a colored or fluorescent dye. However, such treatments generally result in the death of the specimen, thus preventing the ability to study a single population repeatedly over a long time period.

Original languageEnglish (US)
Title of host publicationLabel-Free Biosensors
Subtitle of host publicationTechniques and Applications
PublisherCambridge University Press
Pages1-28
Number of pages28
ISBN (Electronic)9780511626531
ISBN (Print)9780521884532
DOIs
StatePublished - Jan 1 2009

Fingerprint

Biosensing Techniques
Biosensors
Labels
Cells
Chemical compounds
Biomolecules
Tumor Biomarkers
Fluorescent Dyes
Oligomers
Pharmaceutical Preparations
Assays
Bacteria
Blood
Molecular weight
Peptides
Molecules
Fluids
Water
DNA
Enzymes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cunningham, B. T. (2009). Label-free optical biosensors: An introduction. In Label-Free Biosensors: Techniques and Applications (pp. 1-28). Cambridge University Press. https://doi.org/10.1017/CBO9780511626531.003

Label-free optical biosensors : An introduction. / Cunningham, Brian T.

Label-Free Biosensors: Techniques and Applications. Cambridge University Press, 2009. p. 1-28.

Research output: Chapter in Book/Report/Conference proceedingChapter

Cunningham, BT 2009, Label-free optical biosensors: An introduction. in Label-Free Biosensors: Techniques and Applications. Cambridge University Press, pp. 1-28. https://doi.org/10.1017/CBO9780511626531.003
Cunningham BT. Label-free optical biosensors: An introduction. In Label-Free Biosensors: Techniques and Applications. Cambridge University Press. 2009. p. 1-28 https://doi.org/10.1017/CBO9780511626531.003
Cunningham, Brian T. / Label-free optical biosensors : An introduction. Label-Free Biosensors: Techniques and Applications. Cambridge University Press, 2009. pp. 1-28
@inbook{8205ccfb104c4d078029284e0d8ab8cb,
title = "Label-free optical biosensors: An introduction",
abstract = "The ability to study interactions among biomolecules, to observe the activity of cells, and to detect analytes specifically from bodily fluids, manufacturing processes, or environmental samples are cornerstones of life science research, pharmaceutical discovery, medical diagnosis, and food/water safety assurance. These capabilities and many others are enabled by the ability to perform biochemical and cell-based assays that allow scientists to ask basic questions about whether one analyte interacts with another, how strong the binding affinity is between two proteins, whether a chemical compound will affect the proliferation rate of cancer cells, and the concentration of a biomarker for cancer within a patient's blood sample. The development of technology to meet these requirements is challenging because biochemical analytes, which can include drug compounds with molecular weights below 500 Da, DNA oligomers, peptides, enzymes, anti-bodies, and viral particles, are exceedingly small and sometimes present within a test sample at concentrations in the fg/ml to pg/ml concentration range that simultaneously contains thousands of other molecules at concentrations orders of magnitude greater. Larger biochemical analytes – such as bacteria, spores, cells, and cell clusters – are less difficult to observe if they can be stained with a colored or fluorescent dye. However, such treatments generally result in the death of the specimen, thus preventing the ability to study a single population repeatedly over a long time period.",
author = "Cunningham, {Brian T.}",
year = "2009",
month = "1",
day = "1",
doi = "10.1017/CBO9780511626531.003",
language = "English (US)",
isbn = "9780521884532",
pages = "1--28",
booktitle = "Label-Free Biosensors",
publisher = "Cambridge University Press",
address = "United States",

}

TY - CHAP

T1 - Label-free optical biosensors

T2 - An introduction

AU - Cunningham, Brian T.

PY - 2009/1/1

Y1 - 2009/1/1

N2 - The ability to study interactions among biomolecules, to observe the activity of cells, and to detect analytes specifically from bodily fluids, manufacturing processes, or environmental samples are cornerstones of life science research, pharmaceutical discovery, medical diagnosis, and food/water safety assurance. These capabilities and many others are enabled by the ability to perform biochemical and cell-based assays that allow scientists to ask basic questions about whether one analyte interacts with another, how strong the binding affinity is between two proteins, whether a chemical compound will affect the proliferation rate of cancer cells, and the concentration of a biomarker for cancer within a patient's blood sample. The development of technology to meet these requirements is challenging because biochemical analytes, which can include drug compounds with molecular weights below 500 Da, DNA oligomers, peptides, enzymes, anti-bodies, and viral particles, are exceedingly small and sometimes present within a test sample at concentrations in the fg/ml to pg/ml concentration range that simultaneously contains thousands of other molecules at concentrations orders of magnitude greater. Larger biochemical analytes – such as bacteria, spores, cells, and cell clusters – are less difficult to observe if they can be stained with a colored or fluorescent dye. However, such treatments generally result in the death of the specimen, thus preventing the ability to study a single population repeatedly over a long time period.

AB - The ability to study interactions among biomolecules, to observe the activity of cells, and to detect analytes specifically from bodily fluids, manufacturing processes, or environmental samples are cornerstones of life science research, pharmaceutical discovery, medical diagnosis, and food/water safety assurance. These capabilities and many others are enabled by the ability to perform biochemical and cell-based assays that allow scientists to ask basic questions about whether one analyte interacts with another, how strong the binding affinity is between two proteins, whether a chemical compound will affect the proliferation rate of cancer cells, and the concentration of a biomarker for cancer within a patient's blood sample. The development of technology to meet these requirements is challenging because biochemical analytes, which can include drug compounds with molecular weights below 500 Da, DNA oligomers, peptides, enzymes, anti-bodies, and viral particles, are exceedingly small and sometimes present within a test sample at concentrations in the fg/ml to pg/ml concentration range that simultaneously contains thousands of other molecules at concentrations orders of magnitude greater. Larger biochemical analytes – such as bacteria, spores, cells, and cell clusters – are less difficult to observe if they can be stained with a colored or fluorescent dye. However, such treatments generally result in the death of the specimen, thus preventing the ability to study a single population repeatedly over a long time period.

UR - http://www.scopus.com/inward/record.url?scp=84896533792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896533792&partnerID=8YFLogxK

U2 - 10.1017/CBO9780511626531.003

DO - 10.1017/CBO9780511626531.003

M3 - Chapter

AN - SCOPUS:84896533792

SN - 9780521884532

SP - 1

EP - 28

BT - Label-Free Biosensors

PB - Cambridge University Press

ER -