Label-free microscopy: A non-invasive new tool to assess gametes and embryo quality

Marcello Rubessa; Matthew B. Wheeler

doi:10.1016/j.theriogenology.2020.01.065

Label-free microscopy: A non-invasive new tool to assess gametes and embryo quality

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Abstract

In PubMed, it is possible to find more than 40,000 papers on embryo evaluation in various species. However, there is no consensus or gold standard method on how to assess their developmental potential. In assisted reproduction the evaluation “problem” is not only limited to embryos but involves the gametes as well. This manuscript provides an overview of some possible applications of label-free microscopy, in particular we describe the potential of the holographic microscopy in the IVF lab. We describe the positive aspects of several currently available microscopy label-free systems. In conclusion, we believe that a next generation of microscopy able to give objective markers for gamete and embryo quality is around the corner.

Original language	English (US)
Pages (from-to)	241-246
Number of pages	6
Journal	Theriogenology
Volume	150
DOIs	https://doi.org/10.1016/j.theriogenology.2020.01.065
State	Published - Jul 1 2020

Keywords

Embryos
Label-free microscopy
Microscopy
Oocytes
Sperm

ASJC Scopus subject areas

Small Animals
Food Animals
Animal Science and Zoology
Equine

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10.1016/j.theriogenology.2020.01.065

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Label-free microscopy: A non-invasive new tool to assess gametes and embryo quality

Abstract

Keywords

ASJC Scopus subject areas

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