Abstract

We show that applying the Laplace operator to a speckle-free quantitative phase image reveals an unprecedented level of detail in cell structure, without the gradient artifacts associated with differential interference contrast microscopy, or photobleaching and phototoxicity limitations common in fluorescence microscopy. This method, referred to as Laplace phase microscopy, is an efficient tool for tracking vesicles and organelles in living cells. The principle is demonstrated by tracking organelles in cardiomyocytes and vesicles in neurites of hippocampal neurons, which to our knowledge are the first label-free diffusion measurements of the organelles in such cells.

Original languageEnglish (US)
Article number026019
JournalJournal of biomedical optics
Volume16
Issue number2
DOIs
StatePublished - Feb 1 2011

Fingerprint

Light interference
organelles
Labels
Microscopic examination
microscopy
interference
Photobleaching
Fluorescence microscopy
Speckle
Neurons
Laplace transformation
Cells
cells
neurons
artifacts
fluorescence
gradients

Keywords

  • Interference microscopy
  • Intrinsic particle tracking
  • Phase measurement
  • Quantitative phase imaging

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Label-free intracellular transport measured by spatial light interference microscopy. / Wang, Zhuo; Millet, Larry; Chan, Vincent; Ding, Huafeng; Gillette, Martha U.; Bashir, Rashid; Popescu, Gabriel.

In: Journal of biomedical optics, Vol. 16, No. 2, 026019, 01.02.2011.

Research output: Contribution to journalArticle

@article{9bc4318aed6e458b9af4409d27e97e4f,
title = "Label-free intracellular transport measured by spatial light interference microscopy",
abstract = "We show that applying the Laplace operator to a speckle-free quantitative phase image reveals an unprecedented level of detail in cell structure, without the gradient artifacts associated with differential interference contrast microscopy, or photobleaching and phototoxicity limitations common in fluorescence microscopy. This method, referred to as Laplace phase microscopy, is an efficient tool for tracking vesicles and organelles in living cells. The principle is demonstrated by tracking organelles in cardiomyocytes and vesicles in neurites of hippocampal neurons, which to our knowledge are the first label-free diffusion measurements of the organelles in such cells.",
keywords = "Interference microscopy, Intrinsic particle tracking, Phase measurement, Quantitative phase imaging",
author = "Zhuo Wang and Larry Millet and Vincent Chan and Huafeng Ding and Gillette, {Martha U.} and Rashid Bashir and Gabriel Popescu",
year = "2011",
month = "2",
day = "1",
doi = "10.1117/1.3549204",
language = "English (US)",
volume = "16",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "2",

}

TY - JOUR

T1 - Label-free intracellular transport measured by spatial light interference microscopy

AU - Wang, Zhuo

AU - Millet, Larry

AU - Chan, Vincent

AU - Ding, Huafeng

AU - Gillette, Martha U.

AU - Bashir, Rashid

AU - Popescu, Gabriel

PY - 2011/2/1

Y1 - 2011/2/1

N2 - We show that applying the Laplace operator to a speckle-free quantitative phase image reveals an unprecedented level of detail in cell structure, without the gradient artifacts associated with differential interference contrast microscopy, or photobleaching and phototoxicity limitations common in fluorescence microscopy. This method, referred to as Laplace phase microscopy, is an efficient tool for tracking vesicles and organelles in living cells. The principle is demonstrated by tracking organelles in cardiomyocytes and vesicles in neurites of hippocampal neurons, which to our knowledge are the first label-free diffusion measurements of the organelles in such cells.

AB - We show that applying the Laplace operator to a speckle-free quantitative phase image reveals an unprecedented level of detail in cell structure, without the gradient artifacts associated with differential interference contrast microscopy, or photobleaching and phototoxicity limitations common in fluorescence microscopy. This method, referred to as Laplace phase microscopy, is an efficient tool for tracking vesicles and organelles in living cells. The principle is demonstrated by tracking organelles in cardiomyocytes and vesicles in neurites of hippocampal neurons, which to our knowledge are the first label-free diffusion measurements of the organelles in such cells.

KW - Interference microscopy

KW - Intrinsic particle tracking

KW - Phase measurement

KW - Quantitative phase imaging

UR - http://www.scopus.com/inward/record.url?scp=79957839893&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79957839893&partnerID=8YFLogxK

U2 - 10.1117/1.3549204

DO - 10.1117/1.3549204

M3 - Article

C2 - 21361703

AN - SCOPUS:79957839893

VL - 16

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 2

M1 - 026019

ER -