Label-free imaging of human breast tissues using coherent anti-Stokes Raman scattering microscopy

Yaliang Yang, Liang Gao, Zhiyong Wang, Michael J. Thrall, Pengfei Luo, Kelvin K. Wong, Stephen T.C. Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Breast cancer is a common disease in women. Current imaging and diagnostic methods for breast cancer confront several limitations, like time-consuming, invasive and with a high cost. Alternative strategies are in high demand to alleviate patients' trauma and lower medical expenses. Coherent anti-Stokes Raman scattering (CARS) imaging technique offers many advantages, including label-free, sub-wavelength spatial resolution and video-rate imaging speed. Therefore, it has been demonstrated as a powerful tool for various biomedical applications. In this study, we present a label-free fast imaging method to identify breast cancer and its subtypes using CARS microscopy. Human breast tissues, including normal, benign and invasive carcinomas, were imaged ex vivo using a custom-built CARS microscope. Compared with results from corresponding hematoxylin and eosin (H&E) stains, the CARS technique has demonstrated its capability in identifying morphological features in a similar way as in H&E stain. These features can be used to distinguish breast cancer from normal and benign tissues, and further separate cancer subtypes from each other. Our pilot study suggests that CARS microscopy could be used as a routine examination tool to characterize breast cancer ex vivo. Moreover, its label-free and fast imaging properties render this technique as a promising approach for in vivo and real-time imaging and diagnosis of breast cancer.

Original languageEnglish (US)
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XI
DOIs
StatePublished - 2011
EventMultiphoton Microscopy in the Biomedical Sciences XI - San Francisco, CA, United States
Duration: Jan 23 2011Jan 25 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7903
ISSN (Print)1605-7422

Other

OtherMultiphoton Microscopy in the Biomedical Sciences XI
Country/TerritoryUnited States
CitySan Francisco, CA
Period1/23/111/25/11

Keywords

  • Breast cancer
  • Coherent anti-Stokes Raman scattering microscopy
  • Differential diagnosis
  • Nonlinear optical microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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