Abstract

We introduce photonic crystal enhanced microscopy (PCEM) as a label-free biosensor imaging technique capable of measuring cell surface attachment and attachment modulation. The approach uses a photonic crystal optical resonator surface incorporated into conventional microplate wells and a microscope-based detection instrument that measures shifts in the resonant coupling conditions caused by localized changes in dielectric permittivity at the cell-sensor interface. Four model systems are demonstrated for studying cancer cells, primary cardiac muscle cells, and stem cells. First, HepG2/C3 hepatic carcinoma cells were cultured and observed via PCEM in order to characterize cell adhesion in the context of growth and locomotion. Second, Panc-1 pancreatic cancer cells were used to verify that cell attachment density decreases in response to staurosporine, a drug that induces apoptosis. Third, we used PCEM to confirm the influence of integrin-mediated signaling on primary neonatal cardiomyocyte growth and development. Rounded cardiomyocytes consistently showed decreased cell attachment density as recorded via PCEM, while spreading cells exhibited greater attachment strength as well as increased contractility. Finally, PCEM was used to monitor the morphological changes and extracellular matrix remodeling of porcine adipose-derived stem cells subjected to a forced differentiation protocol. Each of these experiments yielded information regarding cell attachment density without the use of potentially cytotoxic labels, enabling study of the same cells for up to several days.

Original languageEnglish (US)
Pages (from-to)3608-3615
Number of pages8
JournalAnalyst
Volume136
Issue number18
DOIs
StatePublished - Sep 21 2011

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Optics and Photonics
Photonic crystals
Labels
microscopy
Microscopy
Microscopic examination
crystal
Imaging techniques
Cardiac Myocytes
Cell Count
Cells
Stem cells
cancer
Stem Cells
stem
Crystal resonators
Optical resonators
Staurosporine
permittivity
microplate

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Label-free imaging of cell attachment with photonic crystal enhanced microscopy. / Lidstone, Erich A.; Chaudhery, Vikram; Kohl, Anja; Chan, Vincent; Wolf-Jensen, Tor; Schook, Lawrence B.; Bashir, Rashid; Cunningham, Brian T.

In: Analyst, Vol. 136, No. 18, 21.09.2011, p. 3608-3615.

Research output: Contribution to journalArticle

Lidstone EA, Chaudhery V, Kohl A, Chan V, Wolf-Jensen T, Schook LB et al. Label-free imaging of cell attachment with photonic crystal enhanced microscopy. Analyst. 2011 Sep 21;136(18):3608-3615. https://doi.org/10.1039/c1an15171a
Lidstone, Erich A. ; Chaudhery, Vikram ; Kohl, Anja ; Chan, Vincent ; Wolf-Jensen, Tor ; Schook, Lawrence B. ; Bashir, Rashid ; Cunningham, Brian T. / Label-free imaging of cell attachment with photonic crystal enhanced microscopy. In: Analyst. 2011 ; Vol. 136, No. 18. pp. 3608-3615.
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