TY - GEN
T1 - Label-free high-resolution imaging of live cells with deconvolved spatial light interference microscopy
AU - Haldar, Justin P.
AU - Wang, Zhuo
AU - Popescu, Gabriel
AU - Liang, Zhi Pei
PY - 2010
Y1 - 2010
N2 - Spatial light interference microscopy (SLIM) is a powerful new quantitative phase optical imaging technique that can be used for studying live cells without the need for exogenous contrast agents. This paper proposes a novel deconvolution-based approach to reconstructing SLIM data, which dramatically improves the visual quality of the images. The proposed deconvolution formulation is tailored to the physics of SLIM imaging of biological samples, and a new fast algorithm is designed for computationally-efficient image reconstruction in this setting. Simulation and experimental results demonstrate that deconvolution can reduce the width of the point-spread function by at least 20%, and can significantly improve the contrast of high-resolution features. Temporally-resolved SLIM imaging with the high spatial resolution enabled by deconvolution provides new opportunities for studying the dynamics of cellular and sub-cellular processes.
AB - Spatial light interference microscopy (SLIM) is a powerful new quantitative phase optical imaging technique that can be used for studying live cells without the need for exogenous contrast agents. This paper proposes a novel deconvolution-based approach to reconstructing SLIM data, which dramatically improves the visual quality of the images. The proposed deconvolution formulation is tailored to the physics of SLIM imaging of biological samples, and a new fast algorithm is designed for computationally-efficient image reconstruction in this setting. Simulation and experimental results demonstrate that deconvolution can reduce the width of the point-spread function by at least 20%, and can significantly improve the contrast of high-resolution features. Temporally-resolved SLIM imaging with the high spatial resolution enabled by deconvolution provides new opportunities for studying the dynamics of cellular and sub-cellular processes.
UR - http://www.scopus.com/inward/record.url?scp=78650827904&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650827904&partnerID=8YFLogxK
U2 - 10.1109/IEMBS.2010.5627917
DO - 10.1109/IEMBS.2010.5627917
M3 - Conference contribution
C2 - 21097241
AN - SCOPUS:78650827904
SN - 9781424441235
T3 - 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10
SP - 3382
EP - 3385
BT - 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10
T2 - 2010 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10
Y2 - 31 August 2010 through 4 September 2010
ER -