Label-free high-resolution imaging of live cells with deconvolved spatial light interference microscopy

Justin P. Haldar, Zhuo Wang, Gabriel Popescu, Zhi-Pei Liang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Spatial light interference microscopy (SLIM) is a powerful new quantitative phase optical imaging technique that can be used for studying live cells without the need for exogenous contrast agents. This paper proposes a novel deconvolution-based approach to reconstructing SLIM data, which dramatically improves the visual quality of the images. The proposed deconvolution formulation is tailored to the physics of SLIM imaging of biological samples, and a new fast algorithm is designed for computationally-efficient image reconstruction in this setting. Simulation and experimental results demonstrate that deconvolution can reduce the width of the point-spread function by at least 20%, and can significantly improve the contrast of high-resolution features. Temporally-resolved SLIM imaging with the high spatial resolution enabled by deconvolution provides new opportunities for studying the dynamics of cellular and sub-cellular processes.

Original languageEnglish (US)
Title of host publication2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10
Pages3382-3385
Number of pages4
DOIs
StatePublished - Dec 1 2010
Event2010 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10 - Buenos Aires, Argentina
Duration: Aug 31 2010Sep 4 2010

Publication series

Name2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10

Other

Other2010 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10
CountryArgentina
CityBuenos Aires
Period8/31/109/4/10

Fingerprint

Interference Microscopy
Light interference
Deconvolution
Labels
Microscopic examination
Imaging techniques
Light
Computer-Assisted Image Processing
Optical Imaging
Physics
Optical transfer function
Image reconstruction
Contrast Media

ASJC Scopus subject areas

  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Health Informatics

Cite this

Haldar, J. P., Wang, Z., Popescu, G., & Liang, Z-P. (2010). Label-free high-resolution imaging of live cells with deconvolved spatial light interference microscopy. In 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10 (pp. 3382-3385). [5627917] (2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10). https://doi.org/10.1109/IEMBS.2010.5627917

Label-free high-resolution imaging of live cells with deconvolved spatial light interference microscopy. / Haldar, Justin P.; Wang, Zhuo; Popescu, Gabriel; Liang, Zhi-Pei.

2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10. 2010. p. 3382-3385 5627917 (2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Haldar, JP, Wang, Z, Popescu, G & Liang, Z-P 2010, Label-free high-resolution imaging of live cells with deconvolved spatial light interference microscopy. in 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10., 5627917, 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10, pp. 3382-3385, 2010 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10, Buenos Aires, Argentina, 8/31/10. https://doi.org/10.1109/IEMBS.2010.5627917
Haldar JP, Wang Z, Popescu G, Liang Z-P. Label-free high-resolution imaging of live cells with deconvolved spatial light interference microscopy. In 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10. 2010. p. 3382-3385. 5627917. (2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10). https://doi.org/10.1109/IEMBS.2010.5627917
Haldar, Justin P. ; Wang, Zhuo ; Popescu, Gabriel ; Liang, Zhi-Pei. / Label-free high-resolution imaging of live cells with deconvolved spatial light interference microscopy. 2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10. 2010. pp. 3382-3385 (2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10).
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