Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

Grace M. Credo, Xing Su, Kai Wu, Oguz H. Elibol, David J. Liu, Bobby Reddy, Ta Wei Tsai, Brian R. Dorvel, Jonathan S. Daniels, Rashid Bashir, Madoo Varma

Research output: Contribution to journalArticle

Abstract

We introduce a label-free approach for sensing polymerase reactions on deoxyribonucleic acid (DNA) using a chelator-modified silicon-on-insulator field-effect transistor (SOI-FET) that exhibits selective and reversible electrical response to pyrophosphate anions. The chemical modification of the sensor surface was designed to include rolling-circle amplification (RCA) DNA colonies for locally enhanced pyrophosphate (PPi) signal generation and sensors with immobilized chelators for capture and surface-sensitive detection of diffusible reaction by-products. While detecting arrays of enzymatic base incorporation reactions is typically accomplished using optical fluorescence or chemiluminescence techniques, our results suggest that it is possible to develop scalable and portable PPi-specific sensors and platforms for broad biomedical applications such as DNA sequencing and microbe detection using surface-sensitive electrical readout techniques.

Original languageEnglish (US)
Pages (from-to)1351-1362
Number of pages12
JournalAnalyst
Volume137
Issue number6
DOIs
StatePublished - Mar 21 2012

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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    Credo, G. M., Su, X., Wu, K., Elibol, O. H., Liu, D. J., Reddy, B., Tsai, T. W., Dorvel, B. R., Daniels, J. S., Bashir, R., & Varma, M. (2012). Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices. Analyst, 137(6), 1351-1362. https://doi.org/10.1039/c2an15930a