Label-free detection of lactoferrin and beta-2-microglobuin in contrived tear film using a low-cost electrical biosensor chip

Muhammad S. Khan, Ketan Dighe, Zhen Wang, Enrique Daza, Aaron S. Schwartz-Duval, Conor P. Rowley, Isaac A. Calvillo, Santosh K. Misra, Leanne T. Labriola, Dipanjan Pan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we present a biosensor chip to study two important biomarkers, lactoferrin (LCF) and beta-2-microglubin (B2M) present in our tear fluid. LCF and B2M have direct relevance with dry eye syndrome and diabetic retinopathy in patients respectively. We have developed a multi-layer paper-based biosensor chip connected with handheld printed circuit board (PCB) to monitor the effectiveness of B2M and LCF in contrived tear film (TF) with a wide range of detection (RoD) from 0.001 to 1ng/mL (R2=0.9886) and 1 to 10mg/mL (R2=0.9554) respectively. Multilayer biosensor is composed of graphene nanoplatelet and diblock-co-polymer coated on a filter paper followed by deposition of micro-Au electrodes. Anti-LCF and anti-B2M antibodies were immobilized via 3,3′-dithiodipropionic acid di(N-hydroxysuccinimide ester (DTSP) as a self-assembled monolayer (SAM) on Au. In addition to electrical measurements in real-time setup, Raman spectral analysis and SEM were utilized to characterize the sensor surface before and after testing the relevant proteins. We anticipate that the fabricated sensor chip has promising application to study dry-eye-disease (DED) and diabetes in Point-of-care (POC) setting.

Original languageEnglish (US)
Title of host publication2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages72-75
Number of pages4
ISBN (Electronic)9781538613924
DOIs
StatePublished - Dec 19 2017
Event2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017 - Bethesda, United States
Duration: Nov 6 2017Nov 8 2017

Publication series

Name2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
Volume2017-December

Other

Other2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
CountryUnited States
CityBethesda
Period11/6/1711/8/17

ASJC Scopus subject areas

  • Health Informatics
  • Instrumentation
  • Health(social science)
  • Biomedical Engineering

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  • Cite this

    Khan, M. S., Dighe, K., Wang, Z., Daza, E., Schwartz-Duval, A. S., Rowley, C. P., Calvillo, I. A., Misra, S. K., Labriola, L. T., & Pan, D. (2017). Label-free detection of lactoferrin and beta-2-microglobuin in contrived tear film using a low-cost electrical biosensor chip. In 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017 (pp. 72-75). (2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017; Vol. 2017-December). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/HIC.2017.8227587