Label-free biosensor arrays based on silicon ring resonators and high-speed optical scanning instrumentation

Muzammil Iqbal, Martin A. Gleeson, Bradley Spaugh, Frank Tybor, William G. Gunn, Michael Hochberg, Tom Baehr-Jones, Ryan C. Bailey, L. Cary Gunn

Research output: Contribution to journalArticlepeer-review

Abstract

A platform for performing rapid, real-time binding assays on sensor arrays based on silicon ring resonators is presented in this paper. An array of 32 sensors is interrogated simultaneously. Using eight sensors as controls, 24 simultaneous binding curves are produced. The bulk refractive index sensitivity of the system was demonstrated down to 7.6 × 10-7 and sensor-to-sensor variability is 3.9%. Using an 8-min incubation, real-time binding was observed over 8-logs of concentration down to 60fM using immobilized biotin to capture streptavidin diluted in bovine serum albumin solution. Multiplexing in complex media is demonstrated with two DNA oligonucleotide probes. Time to result and repeatability are demonstrated to be adequate for clinical applications.

Original languageEnglish (US)
Article number5437305
Pages (from-to)654-661
Number of pages8
JournalIEEE Journal on Selected Topics in Quantum Electronics
Volume16
Issue number3
DOIs
StatePublished - May 2010

Keywords

  • Optical biosensing
  • Refractometric sensor
  • Resonant cavity sensors
  • Ring resonator sensor

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

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