Label-free biosensor arrays based on silicon ring resonators and high-speed optical scanning instrumentation

Muzammil Iqbal; Martin A. Gleeson; Bradley Spaugh; Frank Tybor; William G. Gunn; Michael Hochberg; Tom Baehr-Jones; Ryan C. Bailey; L. Cary Gunn

doi:10.1109/JSTQE.2009.2032510

Label-free biosensor arrays based on silicon ring resonators and high-speed optical scanning instrumentation

Muzammil Iqbal, Martin A. Gleeson, Bradley Spaugh, Frank Tybor, William G. Gunn, Michael Hochberg, Tom Baehr-Jones, Ryan C. Bailey, L. Cary Gunn

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

A platform for performing rapid, real-time binding assays on sensor arrays based on silicon ring resonators is presented in this paper. An array of 32 sensors is interrogated simultaneously. Using eight sensors as controls, 24 simultaneous binding curves are produced. The bulk refractive index sensitivity of the system was demonstrated down to 7.6 × 10^-7 and sensor-to-sensor variability is 3.9%. Using an 8-min incubation, real-time binding was observed over 8-logs of concentration down to 60fM using immobilized biotin to capture streptavidin diluted in bovine serum albumin solution. Multiplexing in complex media is demonstrated with two DNA oligonucleotide probes. Time to result and repeatability are demonstrated to be adequate for clinical applications.

Original language	English (US)
Article number	5437305
Pages (from-to)	654-661
Number of pages	8
Journal	IEEE Journal on Selected Topics in Quantum Electronics
Volume	16
Issue number	3
DOIs	https://doi.org/10.1109/JSTQE.2009.2032510
State	Published - May 2010

Keywords

Optical biosensing
Refractometric sensor
Resonant cavity sensors
Ring resonator sensor

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Electrical and Electronic Engineering

Online availability

10.1109/JSTQE.2009.2032510

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Iqbal, M., Gleeson, M. A., Spaugh, B., Tybor, F., Gunn, W. G., Hochberg, M., Baehr-Jones, T., Bailey, R. C., & Gunn, L. C. (2010). Label-free biosensor arrays based on silicon ring resonators and high-speed optical scanning instrumentation. IEEE Journal on Selected Topics in Quantum Electronics, 16(3), 654-661. Article 5437305. https://doi.org/10.1109/JSTQE.2009.2032510

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title = "Label-free biosensor arrays based on silicon ring resonators and high-speed optical scanning instrumentation",

abstract = "A platform for performing rapid, real-time binding assays on sensor arrays based on silicon ring resonators is presented in this paper. An array of 32 sensors is interrogated simultaneously. Using eight sensors as controls, 24 simultaneous binding curves are produced. The bulk refractive index sensitivity of the system was demonstrated down to 7.6 × 10-7 and sensor-to-sensor variability is 3.9%. Using an 8-min incubation, real-time binding was observed over 8-logs of concentration down to 60fM using immobilized biotin to capture streptavidin diluted in bovine serum albumin solution. Multiplexing in complex media is demonstrated with two DNA oligonucleotide probes. Time to result and repeatability are demonstrated to be adequate for clinical applications.",

keywords = "Optical biosensing, Refractometric sensor, Resonant cavity sensors, Ring resonator sensor",

author = "Muzammil Iqbal and Gleeson, {Martin A.} and Bradley Spaugh and Frank Tybor and Gunn, {William G.} and Michael Hochberg and Tom Baehr-Jones and Bailey, {Ryan C.} and Gunn, {L. Cary}",

note = "Funding Information: Dr. Bailey was the recipient of the 2007 Inaugural Director{\textquoteright}s New Innovator Award from the National Institute of Health.",

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doi = "10.1109/JSTQE.2009.2032510",

language = "English (US)",

volume = "16",

pages = "654--661",

journal = "IEEE Journal on Selected Topics in Quantum Electronics",

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AU - Iqbal, Muzammil

AU - Gleeson, Martin A.

AU - Spaugh, Bradley

AU - Tybor, Frank

AU - Gunn, William G.

AU - Hochberg, Michael

AU - Baehr-Jones, Tom

AU - Bailey, Ryan C.

AU - Gunn, L. Cary

N1 - Funding Information: Dr. Bailey was the recipient of the 2007 Inaugural Director’s New Innovator Award from the National Institute of Health.

PY - 2010/5

Y1 - 2010/5

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AB - A platform for performing rapid, real-time binding assays on sensor arrays based on silicon ring resonators is presented in this paper. An array of 32 sensors is interrogated simultaneously. Using eight sensors as controls, 24 simultaneous binding curves are produced. The bulk refractive index sensitivity of the system was demonstrated down to 7.6 × 10-7 and sensor-to-sensor variability is 3.9%. Using an 8-min incubation, real-time binding was observed over 8-logs of concentration down to 60fM using immobilized biotin to capture streptavidin diluted in bovine serum albumin solution. Multiplexing in complex media is demonstrated with two DNA oligonucleotide probes. Time to result and repeatability are demonstrated to be adequate for clinical applications.

KW - Optical biosensing

KW - Refractometric sensor

KW - Resonant cavity sensors

KW - Ring resonator sensor

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Label-free biosensor arrays based on silicon ring resonators and high-speed optical scanning instrumentation

Abstract

Keywords

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