Label-free assays on the BIND system

Brian T. Cunningham, Peter Li, Stephen Schulz, Bo Lin, Cheryl Baird, John Gerstenmaier, Christine Genick, Frank Wang, Eric Fine, Lance Laing

Research output: Contribution to journalArticle

Abstract

Screening of biochemical interactions becomes simpler, less expensive, and more accurate when labels, such as fluorescent dyes, radioactive markers, and colorimetric reactions, are not required to quantify detected material. SRU Biosystems has developed a biosensor technology that is manufactured on continuous sheets of plastic film and incorporated into standard microplates and microarray slides to enable label-free assays to be performed with high throughput, high sensitivity, and low cost per assay. The biosensor incorporates a narrowband guided-mode resonance reflectance filter, in which the reflected color is modulated by the attachment/detachment of biochemical material to the surface. The technology offers 4 orders of linear dynamic range and uniformity within a plate, with a coefficient of variation of 2.5%. Using conventional biochemical immobilization surface chemistries, a wide range of assay applications are enabled. Small molecule screening, cell proliferation/ cytotoxicity, enzyme activity screening, protein-protein interaction, and cell membrane receptor expression are among the applications demonstrated.

Original languageEnglish (US)
Pages (from-to)481-490
Number of pages10
JournalJournal of Biomolecular Screening
Volume9
Issue number6
DOIs
StatePublished - Sep 1 2004
Externally publishedYes

Keywords

  • BIND
  • Biomolecular Interaction Detection system
  • Biosensors
  • Label-free detection
  • Pharmaceutical screening

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Biotechnology
  • Biochemistry
  • Molecular Biology

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