TY - JOUR
T1 - L-malic Acid Production from Xylose by Engineered Saccharomyces Cerevisiae
AU - Kang, Nam Kyu
AU - Lee, Jae Won
AU - Ort, Donald R.
AU - Jin, Yong Su
N1 - Funding Information:
This study was funded by the DOE Center for Advanced Bioenergy and Bioproducts Innovation (U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research under Award Number DE‐SC0018420). Any opinions, findings, and conclusions or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the U.S. Department of Energy.
Publisher Copyright:
© 2021 Wiley-VCH GmbH
PY - 2022/3
Y1 - 2022/3
N2 - L-malic acid is widely used in the food, chemical, and pharmaceutical industries. Here, we report on production of malic acid from xylose, the second most abundant sugar in lignocellulosic hydrolysates, by engineered Saccharomyces cerevisiae. To enable malic acid production in a xylose-assimilating S. cerevisiae, we overexpressed PYC1 and PYC2, coding for pyruvate carboxylases, a truncated MDH3 coding for malate dehydrogenase, and SpMAE1, coding for a Schizosaccharomyces pombe malate transporter. Additionally, both the ethanol and glycerol-producing pathways were blocked to enhance malic acid production. The resulting strain produced malic acid from both glucose and xylose, but it produced much higher titers of malic acid from xylose than glucose. Interestingly, the engineered strain had higher malic acid yield from lower concentrations (10 g L
‒1) of xylose, with no ethanol production, than from higher xylose concentrations (20 and 40 g L
‒1). As such, a fed-batch culture maintaining xylose concentrations at low levels was conducted and 61.2 g L
‒1 of malic acid was produced, with a productivity of 0.32 g L
‒1 h. These results represent successful engineering of S. cerevisiae for the production of malic acid from xylose, confirming that that xylose offers the efficient production of various biofuels and chemicals by engineered S. cerevisiae.
AB - L-malic acid is widely used in the food, chemical, and pharmaceutical industries. Here, we report on production of malic acid from xylose, the second most abundant sugar in lignocellulosic hydrolysates, by engineered Saccharomyces cerevisiae. To enable malic acid production in a xylose-assimilating S. cerevisiae, we overexpressed PYC1 and PYC2, coding for pyruvate carboxylases, a truncated MDH3 coding for malate dehydrogenase, and SpMAE1, coding for a Schizosaccharomyces pombe malate transporter. Additionally, both the ethanol and glycerol-producing pathways were blocked to enhance malic acid production. The resulting strain produced malic acid from both glucose and xylose, but it produced much higher titers of malic acid from xylose than glucose. Interestingly, the engineered strain had higher malic acid yield from lower concentrations (10 g L
‒1) of xylose, with no ethanol production, than from higher xylose concentrations (20 and 40 g L
‒1). As such, a fed-batch culture maintaining xylose concentrations at low levels was conducted and 61.2 g L
‒1 of malic acid was produced, with a productivity of 0.32 g L
‒1 h. These results represent successful engineering of S. cerevisiae for the production of malic acid from xylose, confirming that that xylose offers the efficient production of various biofuels and chemicals by engineered S. cerevisiae.
KW - C4-dicarboxylic acids
KW - malic acid
KW - metabolic engineering
KW - Saccharomyces cerevisiae
KW - xylose
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U2 - 10.1002/biot.202000431
DO - 10.1002/biot.202000431
M3 - Article
C2 - 34390209
AN - SCOPUS:85113300503
SN - 1860-6768
VL - 17
JO - Biotechnology Journal
JF - Biotechnology Journal
IS - 3
M1 - 2000431
ER -