L-Fucose production by engineered Escherichia coli

Jing Jing Liu, Jae Won Lee, Eun Ju Yun, Sang Min Jung, Jin Ho Seo, Yong Su Jin

Research output: Contribution to journalArticle

Abstract

L-Fucose (6-deoxy-L-galactose) is a major constituent of glycans and glycolipids in mammals. Fucosylation of glycans can confer unique functional properties and may be an economical way to manufacture L-fucose. Research can extract L-fucose directly from brown algae, or by enzymatic hydrolysis of L-fucose-rich microbial exopolysaccharides. However, these L-fucose production methods are not economical or scalable for various applications. We engineered an Escherichia coli strain to produce L-fucose. Specifically, we modified the strain genome to eliminate endogenous L-fucose and lactose metabolism, produce 2′-fucosyllactose (2′-FL), and to liberate L-fucose from 2′-FL. This E. coli strain produced 16.7 g/L of L-fucose with productivity of 0.1 g·L −1 ·h −1 in a fed-batch fermentation. This study presents an efficient one-pot biosynthesis strategy to produce a monomeric form of L-fucose by microbial fermentation, making large-scale industrial production of L-fucose feasible.

Original languageEnglish (US)
Pages (from-to)904-911
Number of pages8
JournalBiotechnology and bioengineering
Volume116
Issue number4
DOIs
StatePublished - Apr 2019

Keywords

  • 2′-fucosyllactose
  • Escherichia coli
  • L-fucose
  • one-pot biosynthesis
  • α-L-fucosidase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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  • Cite this

    Liu, J. J., Lee, J. W., Yun, E. J., Jung, S. M., Seo, J. H., & Jin, Y. S. (2019). L-Fucose production by engineered Escherichia coli. Biotechnology and bioengineering, 116(4), 904-911. https://doi.org/10.1002/bit.26907