Abstract
L-Fucose (6-deoxy-L-galactose) is a major constituent of glycans and glycolipids in mammals. Fucosylation of glycans can confer unique functional properties and may be an economical way to manufacture L-fucose. Research can extract L-fucose directly from brown algae, or by enzymatic hydrolysis of L-fucose-rich microbial exopolysaccharides. However, these L-fucose production methods are not economical or scalable for various applications. We engineered an Escherichia coli strain to produce L-fucose. Specifically, we modified the strain genome to eliminate endogenous L-fucose and lactose metabolism, produce 2′-fucosyllactose (2′-FL), and to liberate L-fucose from 2′-FL. This E. coli strain produced 16.7 g/L of L-fucose with productivity of 0.1 g·L −1 ·h −1 in a fed-batch fermentation. This study presents an efficient one-pot biosynthesis strategy to produce a monomeric form of L-fucose by microbial fermentation, making large-scale industrial production of L-fucose feasible.
Original language | English (US) |
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Pages (from-to) | 904-911 |
Number of pages | 8 |
Journal | Biotechnology and bioengineering |
Volume | 116 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2019 |
Keywords
- 2′-fucosyllactose
- Escherichia coli
- L-fucose
- one-pot biosynthesis
- α-L-fucosidase
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology