L-Arginine protects ovine intestinal epithelial cells from lipopolysaccharide-induced intestinal barrier injury

Hao Zhang, Yaqian Jin, Along Peng, Shuang Guo, Juan J. Loor, Hongrong Wang

Research output: Contribution to journalArticlepeer-review

Abstract

This research aims to explore the effect of L-arginine (Arg) on ovine intestinal epithelial cells (IOECs) from lipopolysaccharide (LPS)-induced intestinal barrier injury. IOECs were incubated for 16 h and classified as 4 groups with the replacement of various mediums, including (1) control (Con), Arg-free Dulbecco's modified Eagle's F12 Ham medium (DMEM); (2) Arg treatment, Arg-free DMEM supplemented with 100 μM Arg; (3) LPS treatment, Arg-free DMEM supplemented with 10 μg/mL LPS; (4) LPS with Arg treatment, Arg-free DMEM supplemented with both 10 μg/mL LPS and 100 μM Arg. Arg supplementation to LPS-treated IOECs resulted in lower (P < 0.05) relative Toll-like receptor 4 (TLR4) protein level. Arg supplementation to LPS-treated IOECs resulted in up-regulated (P < 0.05) zonula occludens-1 (ZO-1), peptide transporter 1 (PEPT1) protein levels, and the ratio of phosphorylated mechanistic target of rapamycin (phospho-mTOR)-to-mTOR. The results show that Arg protects IOECs from LPS induced intestinal barrier injury.

Original languageEnglish (US)
Pages (from-to)1067-1084
Number of pages18
JournalFood and Agricultural Immunology
Volume30
Issue number1
DOIs
StatePublished - Jan 1 2019

Keywords

  • barrier functions
  • intestinal epithelial cells
  • L-arginine
  • lipopolysaccharide

ASJC Scopus subject areas

  • Food Science
  • Immunology
  • Agronomy and Crop Science

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