Kinetics of electron and proton transfer during the reaction of wild type and helix VI mutants of cytochrome bo3 with oxygen

Margareta Svensson-Ek, Jeffrey W. Thomas, Robert B Gennis, Thomas Nilsson, Peter Brzezinski

Research output: Contribution to journalArticlepeer-review

Abstract

Site-directed mutagenesis was used to investigate the mechanism of electron and proton transfer in the ubiquinol oxidase, cytochrome bo3, from Escherichia coli. The reaction between the fully reduced form of the enzyme and dioxygen was studied using the flow-flash method. After rapid mixing of CO-bound enzyme with an O2-containing solution, CO was photodissociated, and the subsequent electron- and proton-transfer reactions were measured spectrophotometrically, the latter using a pH-indicator dye. In the wild- type, pure bo3 enzyme, without bound quinones, we observed a single kinetic phase with a rate constant of about 2.4 x 104 S-1, associated with formation of the ferryl oxygen intermediate, followed by proton uptake from solution with a rate constant of about 1.2 x 104 s-1. Enzyme in which heme o instead of heme b was incorporated into the low-spin site displayed a slower ferryl formation with a rate constant of about 3.6 x 103 s m. Upon replacement of the acidic residue glutamate 286 in helix VI of subunit I with a nonprotonatable residue, electron transfer was slightly accelerated, and proton uptake was impaired. Mutations of other residues in the vicinity of E286 also resulted in a dramatic decrease of proton uptake, suggesting that the environment of this residue is important for efficient proton transfer. In the closely related cytochrome aa3 from P. denitrificans, the corresponding residue (E278) has been suggested to be part of a proton- transfer pathway [Iwata, S., Ostermeier, C., Ludwig, B., and Michel, H. (1995) Nature 376, 660-669]. The results are discussed in terms of a model for electron-proton coupling during dioxygen reduction.

Original languageEnglish (US)
Pages (from-to)13673-13680
Number of pages8
JournalBiochemistry
Volume35
Issue number42
DOIs
StatePublished - Jan 1 1996

ASJC Scopus subject areas

  • Biochemistry

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