Kinetics of circulating progenitor cell mobilization during submaximal exercise

Grace M. Niemiro, Justin Parel, Joseph Beals, Stephan Van Vliet, Scott A. Paluska, Daniel R. Moore, Nicholas A. Burd, Michael De Lisio

Research output: Contribution to journalArticle

Abstract

Circulating progenitor cells (CPCs) are a heterogeneous population of stem/progenitor cells in peripheral blood that includes hematopoietic stem and progenitor cells (HSPCs and HSCs), endothelial progenitor cells (EPCs), and mesenchymal stem cells (MSCs) that are involved in tissue repair and adaptation. CPC mobilization during exercise remains uncharacterized in young adults. The purpose of this study was to investigate the kinetics of CPC mobilization during and after submaximal treadmill running and their relationship to mobilization factors. Seven men [age ± 25.3 ± 2.4 yr, body mass index ± 23.5 ± 1.0 kg/m2, peak O2 uptake (V? o2peak) ± 60.9 ± 2.74 ml·kg-1·min-1] ran on a treadmill for 60 min at 70% V ? O2peak. Blood sampling occurred before (Pre), during [20 min (20e), 40 min (40e), 60 min (60e)], and after exercise [15 min (15p), 60 min (60p), 120 min (120p)] for quantification of CPCs (CD34+), HSPCs (CD34+/CD45low), HSCs (CD34+/CD45low/CD38-), CD34+ MSCs (CD45-/CD34+/CD31-/CD105+), CD34- MSCs (CD45-/CD34-/CD31-/CD105-), and EPCs (CD45-/CD34+/CD31+) via flow cytometry. CPC concentration increased compared with Pre at 20e and 40e (2.7- and 2.4-fold, respectively, P < 0.05). HSPCs and HSCs increased at 20e compared with 60p (2.7- and 2.8-fold, respectively, P < 0.05), whereas EPCs and both MSC populations did not change. CXC chemokine ligand (CXCL) 12 (1.5-fold; P < 0.05) and stem cell factor (1.3-fold; P < 0.05) were increased at 40e and remained elevated postexercise. The peak increase in CPCs was positively correlated to concentration of endothelial cells during exercise with no relationship to CXCL12 and SCF. Our data show the kinetics of progenitor cell mobilization during exercise that could provide insight into cellular mediators of exerciseinduced adaptations, and have implication for the use of exercise as an adjuvant therapy for CPC collection in hematopoietic stem cell transplant. NEW & NOTEWORTHY Using a comprehensive evaluation of circulating progenitor cells (CPCs), we show that CPC mobilization during exercise is related to tissue damage, and not plasma concentrations of CXC chemokine ligand 12 and stem cell factor. These data have implications for the use of exercise interventions as adjuvant therapy for CPC mobilization in the context of hematopoietic stem cell transplant and also support the role of mobilized progenitor cells as cellular mediators of systemic adaptations to exercise. hematopoietic stem and progenitor cell; mesenchymal stem cell; endothelial progenitor cell; stem cell factor; CXCL12; hematopoietic stem cell transplant.

Original languageEnglish (US)
Pages (from-to)675-682
Number of pages8
JournalJournal of Applied Physiology
Volume122
Issue number3
DOIs
StatePublished - Mar 2017

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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