Abstract
Small RNAs (sRNAs) are important gene regulators in bacteria. Many sRNAs act post-transcriptionally by affecting translation and degradation of the target mRNAs upon base-pairing interactions. Here we present a general approach combining imaging and mathematical modeling to determine kinetic parameters at different levels of sRNA-mediated gene regulation that contribute to overall regulation efficacy. Our data reveal that certain sRNAs previously characterized as post-transcriptional regulators can regulate some targets co-transcriptionally, leading to a revised model that sRNA-mediated regulation can occur early in an mRNA's lifetime, as soon as the sRNA binding site is transcribed. This co-transcriptional regulation is likely mediated by Rho-dependent termination when transcription-coupled translation is reduced upon sRNA binding. Our data also reveal several important kinetic steps that contribute to the differential regulation of mRNA targets by an sRNA. Particularly, binding of sRNA to the target mRNA may dictate the regulation hierarchy observed within an sRNA regulon.
Original language | English (US) |
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Article number | 109764 |
Journal | Cell Reports |
Volume | 36 |
Issue number | 13 |
DOIs | |
State | Published - Sep 28 2021 |
Keywords
- bacterial small RNA
- fluorescence microscopy
- gene regulation
- mathematical modeling
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology