Kinetic isotope effects in the oxidation of arachidonic acid by soybean lipoxygenase-1

Cyril Jacquot; Sheng Peng; Wilfred A. van der Donk

doi:10.1016/j.bmcl.2008.08.108

Kinetic isotope effects in the oxidation of arachidonic acid by soybean lipoxygenase-1

Cyril Jacquot, Sheng Peng, Wilfred A. van der Donk

Research output: Contribution to journal › Article › peer-review

Abstract

The reaction of soybean lipoxygenase-1 with linoleic acid has been extensively studied and displays very large kinetic isotope effects. In this work, substrate and solvent kinetic isotope effects as well as the viscosity dependence of the oxidation of arachidonic acid were investigated. The hydrogen atom abstraction step was rate-determining at all temperatures, but was partially masked by a viscosity-dependent step at ambient temperatures. The observed KIEs on k_cat were large (∼100 at 25 °C).

Original language	English (US)
Pages (from-to)	5959-5962
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	18
Issue number	22
DOIs	https://doi.org/10.1016/j.bmcl.2008.08.108
State	Published - Nov 15 2008

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2008.08.108

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Kinetic isotope effects in the oxidation of arachidonic acid by soybean lipoxygenase-1

Abstract

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