Kinetic isotope effects in the oxidation of arachidonic acid by soybean lipoxygenase-1

Cyril Jacquot, Sheng Peng, Wilfred A. van der Donk

Research output: Contribution to journalArticle

Abstract

The reaction of soybean lipoxygenase-1 with linoleic acid has been extensively studied and displays very large kinetic isotope effects. In this work, substrate and solvent kinetic isotope effects as well as the viscosity dependence of the oxidation of arachidonic acid were investigated. The hydrogen atom abstraction step was rate-determining at all temperatures, but was partially masked by a viscosity-dependent step at ambient temperatures. The observed KIEs on kcat were large (∼100 at 25 °C).

Original languageEnglish (US)
Pages (from-to)5959-5962
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume18
Issue number22
DOIs
StatePublished - Nov 15 2008

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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