@article{80defc5f2fe940fc82d39297cc2b787c,
title = "Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model",
abstract = "Countermeasures to prevent and treat coronavirus disease 2019 (COVID-19) are a global health priority. We enrolled a cohort of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–recovered participants, developed neutralization assays to investigate antibody responses, adapted our high-throughput antibody generation pipeline to rapidly screen more than 1800 antibodies, and established an animal model to test protection. We isolated potent neutralizing antibodies (nAbs) to two epitopes on the receptor binding domain (RBD) and to distinct non-RBD epitopes on the spike (S) protein. As indicated by maintained weight and low lung viral titers in treated animals, the passive transfer of a nAb provides protection against disease in high-dose SARS-CoV-2 challenge in Syrian hamsters. The study suggests a role for nAbs in prophylaxis, and potentially therapy, of COVID-19. The nAbs also define protective epitopes to guide vaccine design.",
author = "Rogers, {Thomas F.} and Fangzhu Zhao and Deli Huang and Nathan Beutler and Alison Burns and He, {Wan Ting} and Oliver Limbo and Chloe Smith and Ge Song and Jordan Woehl and Linlin Yang and Abbott, {Robert K.} and Sean Callaghan and Elijah Garcia and Jonathan Hurtado and Mara Parren and Linghang Peng and Sydney Ramirez and James Ricketts and Ricciardi, {Michael J.} and Rawlings, {Stephen A.} and Wu, {Nicholas C.} and Meng Yuan and Smith, {Davey M.} and David Nemazee and Teijaro, {John R.} and Voss, {James E.} and Wilson, {Ian A.} and Raiees Andrabi and Bryan Briney and Elise Landais and Devin Sok and Jardine, {Joseph G.} and Burton, {Dennis R.}",
note = "Funding Information: We thank T. Gilman, A. Salazar, and Biosero for their contribution to high-throughput pipeline generation. We also thank B. Graham and J. S. McLellan for provision of materials. We thank L. Walker for valuable manuscript discussions. We thank all the COVID-19 cohort participants for donating samples. This work was supported by the NIH CHAVD (UM1 AI144462 to I.A.W., B.B., D.S., and D.R.B.), R01 (AI132317 and AI073148 to D.N.), and K99 (AI145762 to R.K.A.) awards; the IAVI Neutralizing Antibody Center; and the Bill and Melinda Gates Foundation (OPP 1170236 to I.A.W. and D.R.B., OPP 1183956 to J.E.V., and OPP1196345/ INV-008813 to D.S. and D.R.B.). This work was also supported by the John and Mary Tu Foundation and the Pendleton Trust. Publisher Copyright: Copyright {\textcopyright} 2020 The Authors,",
year = "2020",
month = aug,
day = "21",
doi = "10.1126/science.abc7520",
language = "English (US)",
volume = "369",
pages = "956--963",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6506",
}