Isolation of <Glu-Asn-Phe-His-Leu-Arg-Pro-NH2 (Antho-RPamide II), a novel, biologically active neuropeptide from sea anemones

Klaus Carstensen, Ian D. McFarlane, Kenneth L. Rinehart, Diane Hudman, Furong Sun, Cornelis J.P. Grimmelikhuijzen

Research output: Contribution to journalArticlepeer-review


Using a radioimmunoassay against the C-terminal sequence Arg-Pro-NH2 (RPamide) we have isolated the neuropeptide <Glu-Asn-Phe-His-Leu-Arg-Pro-NH2 (Antho-RPamide II) from extracts of the sea anemone Anthopleura elegantissima. Addition of low concentrations of Antho-RPamide II to a tentacle preparation of sea anemones inhibited the spontaneous, rhythmic contractions, suggesting that the peptide is a neurotransmitter or neuromodulator.

Original languageEnglish (US)
Pages (from-to)131-135
Number of pages5
Issue number2
StatePublished - 1993


  • Coelenterate
  • Mass spectrometry
  • Neurohormone
  • Neurotransmitter
  • Peptide structure

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience


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