Isolation of intact nuclei from Euglena gracilis

M. J. Lynch; D. E. Buetow

doi:10.1016/0014-4827(75)90113-5

Isolation of intact nuclei from Euglena gracilis

M. J. Lynch, D. E. Buetow

Molecular and Integrative Physiology

Research output: Contribution to journal › Article › peer-review

Abstract

A method for the isolation of nuclei from the lower eukaryote, Euglena gracilis, utilizing MES buffer at pH 5.5 and sodium meta bisulfite is presented. The method takes advantage of the finding that sodium meta bisulfite reversibly alters the density of the nuclei allowing them to be separated from cytoplasmic fragments and unbroken whole cells. Nuclei are obtained in a yield of 25-37 %, appear intact ultrastructurally, and contain acid-soluble proteins in an amount relatively the same as found in higher cell nuclei.

Original language	English (US)
Pages (from-to)	344-348
Number of pages	5
Journal	Experimental Cell Research
Volume	91
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(75)90113-5
State	Published - Mar 15 1975

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Cell Biology

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10.1016/0014-4827(75)90113-5

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title = "Isolation of intact nuclei from Euglena gracilis",

abstract = "A method for the isolation of nuclei from the lower eukaryote, Euglena gracilis, utilizing MES buffer at pH 5.5 and sodium meta bisulfite is presented. The method takes advantage of the finding that sodium meta bisulfite reversibly alters the density of the nuclei allowing them to be separated from cytoplasmic fragments and unbroken whole cells. Nuclei are obtained in a yield of 25-37 %, appear intact ultrastructurally, and contain acid-soluble proteins in an amount relatively the same as found in higher cell nuclei.",

author = "Lynch, {M. J.} and Buetow, {D. E.}",

note = "Funding Information: We thank Dr Robin Leake for helpful suggestions and discussionsa nd Mr Victor Bell for doing the electron microscopy. A portion of the present study has been presentedb efore in abstract form [24]. This research was supported in part by NIH grant HD 03163. M. J. L. was supported by NIH Training Grant GM 619. These data are from a thesis presentedb y M. J. L. in partial fulfillment of the requirements for the degree of Ph.D., at the University of Illinois.",

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doi = "10.1016/0014-4827(75)90113-5",

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AU - Buetow, D. E.

N1 - Funding Information: We thank Dr Robin Leake for helpful suggestions and discussionsa nd Mr Victor Bell for doing the electron microscopy. A portion of the present study has been presentedb efore in abstract form [24]. This research was supported in part by NIH grant HD 03163. M. J. L. was supported by NIH Training Grant GM 619. These data are from a thesis presentedb y M. J. L. in partial fulfillment of the requirements for the degree of Ph.D., at the University of Illinois.

PY - 1975/3/15

Y1 - 1975/3/15

N2 - A method for the isolation of nuclei from the lower eukaryote, Euglena gracilis, utilizing MES buffer at pH 5.5 and sodium meta bisulfite is presented. The method takes advantage of the finding that sodium meta bisulfite reversibly alters the density of the nuclei allowing them to be separated from cytoplasmic fragments and unbroken whole cells. Nuclei are obtained in a yield of 25-37 %, appear intact ultrastructurally, and contain acid-soluble proteins in an amount relatively the same as found in higher cell nuclei.

AB - A method for the isolation of nuclei from the lower eukaryote, Euglena gracilis, utilizing MES buffer at pH 5.5 and sodium meta bisulfite is presented. The method takes advantage of the finding that sodium meta bisulfite reversibly alters the density of the nuclei allowing them to be separated from cytoplasmic fragments and unbroken whole cells. Nuclei are obtained in a yield of 25-37 %, appear intact ultrastructurally, and contain acid-soluble proteins in an amount relatively the same as found in higher cell nuclei.

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Isolation of intact nuclei from Euglena gracilis

Abstract

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