Isolation of a single-stranded plasmid from Clostridium acetobutylicum NCIB 6444

A. Y. Kim, A. A. Vertes, Hans-Peter M Blaschek

Research output: Contribution to journalArticlepeer-review

Abstract

The cryptic plasmid pDM6 was isolated from late exponential-phase cells of Clostridium acetobutylicum NCIB 6444 by either alkaline lysis or electroporation. The application of high voltage electroporation resulted in higher DNA yield than did the alkaline lysis procedure. However, electroporation-induced plasmid release generated high amounts of single-stranded DNA compared with the alkaline lysis procedure, which generated both double-stranded DNA (monomer and dimer forms) and single-stranded DNA.

Original languageEnglish (US)
Pages (from-to)1725-1728
Number of pages4
JournalApplied and Environmental Microbiology
Volume56
Issue number6
StatePublished - 1990

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

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