Isolation of α‐Terpineol Dehydratase from Pseudomonas gladioli

K. R. CADWALLADER, R. J. BRADDOCK, M. E. PARISH

Research output: Contribution to journalArticle

Abstract

An enzyme which converts the citrus by‐product limonene to α‐terpineol was purified 13‐fold. After cell disruption of P. gladioli, α‐terpineol dehydratase (α‐TD) remained associated with a heterogeneous group of particulate material. α‐TD was partially solubilized by extraction with 10 mM HEPES buffer, pH 7.0 containing 2.0% (w/v) Triton X‐100 and 0.5M sodium trichloroacetate. Two soluble forms existed in 1.0% (w/v) Triton X‐100 with apparent molecular weights of 94,500 and 206,500 daltons.

Original languageEnglish (US)
Pages (from-to)241-244
Number of pages4
JournalJournal of food science
Volume57
Issue number1
DOIs
StatePublished - Jan 1992
Externally publishedYes

Keywords

  • citrus products
  • enzymes
  • limonene conversion
  • molecular weight
  • α‐terpineol dehydratase

ASJC Scopus subject areas

  • Food Science

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