TY - JOUR
T1 - Isolation, fractionation and template activity of the continuously-condensed chromatin of Euglena gracilis
AU - Lynch, M. J.
AU - Leake, R. E.
AU - O'Connell, K. M.
AU - Buetow, D. E.
N1 - Funding Information:
These data are taken from a thesisp resentedb y M. J. L. in partial fulfillment of the requirementsf or the degreeo f Ph.D., at the University of Illinois. A portion of the present study has been presented before in abstract form [42]. This researchw as supportedi n part by NIH grant HD 03163.M . J. L. was supported by NIH Training Grant GM 619.
PY - 1975/3/15
Y1 - 1975/3/15
N2 - A technique for isolating whole chromatin from nuclei of the lower eukaryote Euglena gracilis is presented. This chromatin, which appears under the electron microscope as uniformly condensed fibers, can, nevertheless, be subfractionated into distinct heterochromatic and euchromatic fractions. The euchromatin, comprising about 14% of the total DNA of the nucleus, contains over 80 % of the total endogenous RNA polymerase activity measured. The Km for this enzyme is higher than that found for prokaryotes, but falls in the range found for other eukaryotes. Stability constants, calculated from cation-chromatin binding data, suggest that internal carboxyl groups of chromosomal proteins, at least, are involved in the condensation of Euglena chromatin. The relationship between Euglena chromatin and that of higher eukaryotes is discussed.
AB - A technique for isolating whole chromatin from nuclei of the lower eukaryote Euglena gracilis is presented. This chromatin, which appears under the electron microscope as uniformly condensed fibers, can, nevertheless, be subfractionated into distinct heterochromatic and euchromatic fractions. The euchromatin, comprising about 14% of the total DNA of the nucleus, contains over 80 % of the total endogenous RNA polymerase activity measured. The Km for this enzyme is higher than that found for prokaryotes, but falls in the range found for other eukaryotes. Stability constants, calculated from cation-chromatin binding data, suggest that internal carboxyl groups of chromosomal proteins, at least, are involved in the condensation of Euglena chromatin. The relationship between Euglena chromatin and that of higher eukaryotes is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0016813572&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016813572&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(75)90114-7
DO - 10.1016/0014-4827(75)90114-7
M3 - Article
C2 - 805055
AN - SCOPUS:0016813572
SN - 0014-4827
VL - 91
SP - 349
EP - 357
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -