Isolation and structure of the Streptococcus faecalis sex pheromone, cAM373

Masaaki Mori; Hideo Tanaka; Youji Sakagami; Akira Isogai; Masahiko Fujino; Chieko Kitada; Bryan A. White; Florence Y. An; Don B. Clewell; Akinori Suzuki

doi:10.1016/0014-5793(86)81342-4

Isolation and structure of the Streptococcus faecalis sex pheromone, cAM373

Masaaki Mori, Hideo Tanaka, Youji Sakagami, Akira Isogai, Masahiko Fujino, Chieko Kitada, Bryan A. White, Florence Y. An, Don B. Clewell, Akinori Suzuki

Research output: Contribution to journal › Article › peer-review

Abstract

The Streptococcus faecalis sex pheromone, cAM373, which induces a mating response of donor cells harboring plasmid pAM373 and is also produced by Staphylococcus aureus, was isolated and its structure determined. Supernatant from an overnight culture of a recipient strain was subjected to successive purification procedures, and 4.4 μg cAM373 was obtained. The isolated pheromone showed activity at a concentration as low as 5 × 10^-11 M. Sequence analysis indicated that cAM373 was a heptapeptide, H-Ala-Ile-Phe-Ile-Leu-Ala-Ser-OH, and that its M_r was 733. A synthetic replicate of the peptide showed the same biological activity and chromatographic behavior as the native cAM373.

Original language	English (US)
Pages (from-to)	69-72
Number of pages	4
Journal	FEBS Letters
Volume	206
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(86)81342-4
State	Published - Sep 29 1986
Externally published	Yes

Keywords

(Streptococcus faecalis, staphylococcus aureus)
Conjugative plasmid transfer Amino acid sequence
Sex pheromone

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Online availability

10.1016/0014-5793(86)81342-4

Library availability

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title = "Isolation and structure of the Streptococcus faecalis sex pheromone, cAM373",

abstract = "The Streptococcus faecalis sex pheromone, cAM373, which induces a mating response of donor cells harboring plasmid pAM373 and is also produced by Staphylococcus aureus, was isolated and its structure determined. Supernatant from an overnight culture of a recipient strain was subjected to successive purification procedures, and 4.4 μg cAM373 was obtained. The isolated pheromone showed activity at a concentration as low as 5 × 10-11 M. Sequence analysis indicated that cAM373 was a heptapeptide, H-Ala-Ile-Phe-Ile-Leu-Ala-Ser-OH, and that its Mr was 733. A synthetic replicate of the peptide showed the same biological activity and chromatographic behavior as the native cAM373.",

keywords = "(Streptococcus faecalis, staphylococcus aureus), Conjugative plasmid transfer Amino acid sequence, Sex pheromone",

author = "Masaaki Mori and Hideo Tanaka and Youji Sakagami and Akira Isogai and Masahiko Fujino and Chieko Kitada and White, {Bryan A.} and An, {Florence Y.} and Clewell, {Don B.} and Akinori Suzuki",

note = "Funding Information: This work was supportedi n part by a Grant-in-Aid for ScientificR esearch( no.60790127fr)o m the Ministry of Education, Science and Culture of Japan.",

year = "1986",

month = sep,

day = "29",

doi = "10.1016/0014-5793(86)81342-4",

language = "English (US)",

volume = "206",

pages = "69--72",

journal = "FEBS Letters",

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T1 - Isolation and structure of the Streptococcus faecalis sex pheromone, cAM373

AU - Mori, Masaaki

AU - Tanaka, Hideo

AU - Sakagami, Youji

AU - Isogai, Akira

AU - Fujino, Masahiko

AU - Kitada, Chieko

AU - White, Bryan A.

AU - An, Florence Y.

AU - Clewell, Don B.

AU - Suzuki, Akinori

N1 - Funding Information: This work was supportedi n part by a Grant-in-Aid for ScientificR esearch( no.60790127fr)o m the Ministry of Education, Science and Culture of Japan.

PY - 1986/9/29

Y1 - 1986/9/29

N2 - The Streptococcus faecalis sex pheromone, cAM373, which induces a mating response of donor cells harboring plasmid pAM373 and is also produced by Staphylococcus aureus, was isolated and its structure determined. Supernatant from an overnight culture of a recipient strain was subjected to successive purification procedures, and 4.4 μg cAM373 was obtained. The isolated pheromone showed activity at a concentration as low as 5 × 10-11 M. Sequence analysis indicated that cAM373 was a heptapeptide, H-Ala-Ile-Phe-Ile-Leu-Ala-Ser-OH, and that its Mr was 733. A synthetic replicate of the peptide showed the same biological activity and chromatographic behavior as the native cAM373.

AB - The Streptococcus faecalis sex pheromone, cAM373, which induces a mating response of donor cells harboring plasmid pAM373 and is also produced by Staphylococcus aureus, was isolated and its structure determined. Supernatant from an overnight culture of a recipient strain was subjected to successive purification procedures, and 4.4 μg cAM373 was obtained. The isolated pheromone showed activity at a concentration as low as 5 × 10-11 M. Sequence analysis indicated that cAM373 was a heptapeptide, H-Ala-Ile-Phe-Ile-Leu-Ala-Ser-OH, and that its Mr was 733. A synthetic replicate of the peptide showed the same biological activity and chromatographic behavior as the native cAM373.

KW - (Streptococcus faecalis, staphylococcus aureus)

KW - Conjugative plasmid transfer Amino acid sequence

KW - Sex pheromone

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DO - 10.1016/0014-5793(86)81342-4

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VL - 206

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JF - FEBS Letters

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Isolation and structure of the Streptococcus faecalis sex pheromone, cAM373

Abstract

Keywords

ASJC Scopus subject areas

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