Isolation and structure of the Streptococcus faecalis sex pheromone, cAM373

Masaaki Mori, Hideo Tanaka, Youji Sakagami, Akira Isogai, Masahiko Fujino, Chieko Kitada, Bryan A. White, Florence Y. An, Don B. Clewell, Akinori Suzuki

Research output: Contribution to journalArticlepeer-review

Abstract

The Streptococcus faecalis sex pheromone, cAM373, which induces a mating response of donor cells harboring plasmid pAM373 and is also produced by Staphylococcus aureus, was isolated and its structure determined. Supernatant from an overnight culture of a recipient strain was subjected to successive purification procedures, and 4.4 μg cAM373 was obtained. The isolated pheromone showed activity at a concentration as low as 5 × 10-11 M. Sequence analysis indicated that cAM373 was a heptapeptide, H-Ala-Ile-Phe-Ile-Leu-Ala-Ser-OH, and that its Mr was 733. A synthetic replicate of the peptide showed the same biological activity and chromatographic behavior as the native cAM373.

Original languageEnglish (US)
Pages (from-to)69-72
Number of pages4
JournalFEBS Letters
Volume206
Issue number1
DOIs
StatePublished - Sep 29 1986

Keywords

  • (Streptococcus faecalis, staphylococcus aureus)
  • Conjugative plasmid transfer Amino acid sequence
  • Sex pheromone

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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