Isolation and some characterisation of antifreeze protein from the European eelpout Zoarces viviparus

T. F. Sørensen, C. H.C. Cheng, H. Ramløv

Research output: Contribution to journalArticlepeer-review

Abstract

The European eelpout Zoarces viviparus is a common inhabitant in the coastal areas of the eastern Atlantic Ocean and the Baltic region. At least 3 different antifreeze proteins were purified from Z. viviparus serum but more isoforms are most likely present. Two antifreeze proteins with molecular weights of approx. 6.5-7 kDa were characterised and found to share high similarity to the type III antifreeze proteins found in other members of the family Zoarcidae. The antifreeze activity of Z. viviparus antifreeze proteins is concentration dependent and showed a saturation effect when the protein concentration reached 30mg·ml-1 (crude serum) and 8mg·ml-1 (partly purified serum) respectively. Further the antifreeze activity was found to be dependent of the buffer osmolality resulting in increasing thermal hysteresis when buffer osmolality was raised from 0 to 1M.

Original languageEnglish (US)
Pages (from-to)387-399
Number of pages13
JournalCryo-Letters
Volume27
Issue number6
StatePublished - Nov 1 2006

Keywords

  • Antifreeze proteins
  • Characterisation
  • Purification
  • Zoarces viviparus
  • Zoarcidae

ASJC Scopus subject areas

  • Anatomy
  • Agronomy and Crop Science

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