Isolation and characterization of microsatellites in yellow perch (Perca flavescens)

L. Li, H. P. Wang, C. Givens, S. Czesny, B. Brown

Research output: Contribution to journalArticlepeer-review

Abstract

A total of 45 microsatellite loci from yellow perch, Perca flavescens, were isolated and characterized. Among the 45 microsatellite loci, 32 had more than two alleles. A wild population of P. flavescens (n = 48) was used to examine the allele range of the microsatellite loci. Mendelian inheritance of alleles was confirmed by examining the amplified products in pair-mated families. The number of alleles for the 32 polymorphic loci varied from two to 16, and observed heterozygosity ranged between 0.024 (YP79) and 0.979 (YP60). Cross-species polymorphic amplification in four other Percidae species was successful for 22 loci.

Original languageEnglish (US)
Pages (from-to)600-603
Number of pages4
JournalMolecular Ecology Notes
Volume7
Issue number4
DOIs
StatePublished - Jul 1 2007

Keywords

  • Microsatellite
  • Perca flavescens
  • Tetranucleotide
  • Trinucleotide
  • Yellow perch

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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