Isolation and characterization of microsatellite markers for Sabatia campestris (Gentianaceae), an Illinois state-endangered plant: Primer note

Sheila A. Lyons-Sobaski; Jonathan Edward Beever

doi:10.1111/j.1471-8286.2006.01574.x

Isolation and characterization of microsatellite markers for Sabatia campestris (Gentianaceae), an Illinois state-endangered plant: Primer note

Sheila A. Lyons-Sobaski, Jonathan Edward Beever

Animal Sciences

Research output: Contribution to journal › Review article › peer-review

Abstract

Sabatia campestris is a regionally endangered prairie plant. Eight polymorphic loci have been generated for S. campestris (Gentianaceae) using non-enriched and enriched libraries. Polymorphism was quantified from an isolated population from central Illinois yielding mean observed and expected heterozygosities of 0.723 and 0.801, respectively. The number of alleles per locus for this population ranged from three to 25 with a median of 15.5. These genetic markers will be used to determine population genetic structure of Illinois populations as well as to estimate fine-scale gene flow rates.

Original language	English (US)
Pages (from-to)	260-262
Number of pages	3
Journal	Molecular Ecology Notes
Volume	7
Issue number	2
DOIs	https://doi.org/10.1111/j.1471-8286.2006.01574.x
State	Published - Mar 1 2007

Keywords

Conservation
Gentianaceae
Microsatellites
Population genetics
Prairie rose gentian
Sabatia campestris

ASJC Scopus subject areas

Biochemistry
Ecology
Biochemistry, Genetics and Molecular Biology(all)

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10.1111/j.1471-8286.2006.01574.x

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title = "Isolation and characterization of microsatellite markers for Sabatia campestris (Gentianaceae), an Illinois state-endangered plant: Primer note",

abstract = "Sabatia campestris is a regionally endangered prairie plant. Eight polymorphic loci have been generated for S. campestris (Gentianaceae) using non-enriched and enriched libraries. Polymorphism was quantified from an isolated population from central Illinois yielding mean observed and expected heterozygosities of 0.723 and 0.801, respectively. The number of alleles per locus for this population ranged from three to 25 with a median of 15.5. These genetic markers will be used to determine population genetic structure of Illinois populations as well as to estimate fine-scale gene flow rates.",

keywords = "Conservation, Gentianaceae, Microsatellites, Population genetics, Prairie rose gentian, Sabatia campestris",

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year = "2007",

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T1 - Isolation and characterization of microsatellite markers for Sabatia campestris (Gentianaceae), an Illinois state-endangered plant

T2 - Primer note

AU - Lyons-Sobaski, Sheila A.

AU - Beever, Jonathan Edward

PY - 2007/3/1

Y1 - 2007/3/1

N2 - Sabatia campestris is a regionally endangered prairie plant. Eight polymorphic loci have been generated for S. campestris (Gentianaceae) using non-enriched and enriched libraries. Polymorphism was quantified from an isolated population from central Illinois yielding mean observed and expected heterozygosities of 0.723 and 0.801, respectively. The number of alleles per locus for this population ranged from three to 25 with a median of 15.5. These genetic markers will be used to determine population genetic structure of Illinois populations as well as to estimate fine-scale gene flow rates.

AB - Sabatia campestris is a regionally endangered prairie plant. Eight polymorphic loci have been generated for S. campestris (Gentianaceae) using non-enriched and enriched libraries. Polymorphism was quantified from an isolated population from central Illinois yielding mean observed and expected heterozygosities of 0.723 and 0.801, respectively. The number of alleles per locus for this population ranged from three to 25 with a median of 15.5. These genetic markers will be used to determine population genetic structure of Illinois populations as well as to estimate fine-scale gene flow rates.

KW - Conservation

KW - Gentianaceae

KW - Microsatellites

KW - Population genetics

KW - Prairie rose gentian

KW - Sabatia campestris

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DO - 10.1111/j.1471-8286.2006.01574.x

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SP - 260

EP - 262

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

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Isolation and characterization of microsatellite markers for Sabatia campestris (Gentianaceae), an Illinois state-endangered plant: Primer note

Abstract

Keywords

ASJC Scopus subject areas

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