Isolation and characterization of 45 polymorphic microsatellites from the bovine genome

R. Z. Ma; I. Russ; C. Park; D. W. Heyen; J. E. Beever; C. A. Green; H. A. Lewin

doi:10.1111/j.1365-2052.1996.tb01175.x

Isolation and characterization of 45 polymorphic microsatellites from the bovine genome

R. Z. Ma
, I. Russ
, C. Park
, D. W. Heyen
, J. E. Beever
, C. A. Green
, H. A. Lewin

Animal Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A small-insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single-stranded pBluescript DNA carrying (CA)_n/(TG)_n tandem repeats. Approximately 50% of the clones in the enriched library contained (CA)_n repeats or CA-rich sequences. Sequencing of clones selected for (CA)_n repeats resulted in the identification and characterization of 45 (CA)_n polymorphic microsatellites. Genotyping in 9 large paternal half-sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6·3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high-resolution linkage map of bovine genome.

Original language	English (US)
Pages (from-to)	43-47
Number of pages	5
Journal	Animal genetics
Volume	27
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-2052.1996.tb01175.x
State	Published - Feb 1996

Keywords

Bovine gene mapping
Microsatellites
Polymorphism

ASJC Scopus subject areas

Animal Science and Zoology
Genetics

Online availability

10.1111/j.1365-2052.1996.tb01175.x

Library availability

Discover UIUC Full Text

Cite this

@article{93d88404984a4cce947e4a007ed87c45,

title = "Isolation and characterization of 45 polymorphic microsatellites from the bovine genome",

abstract = "A small-insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single-stranded pBluescript DNA carrying (CA)n/(TG)n tandem repeats. Approximately 50\% of the clones in the enriched library contained (CA)n repeats or CA-rich sequences. Sequencing of clones selected for (CA)n repeats resulted in the identification and characterization of 45 (CA)n polymorphic microsatellites. Genotyping in 9 large paternal half-sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6·3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high-resolution linkage map of bovine genome.",

keywords = "Bovine gene mapping, Microsatellites, Polymorphism",

author = "Ma, \{R. Z.\} and I. Russ and C. Park and Heyen, \{D. W.\} and Beever, \{J. E.\} and Green, \{C. A.\} and Lewin, \{H. A.\}",

year = "1996",

month = feb,

doi = "10.1111/j.1365-2052.1996.tb01175.x",

language = "English (US)",

volume = "27",

pages = "43--47",

journal = "Animal genetics",

issn = "0268-9146",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Isolation and characterization of 45 polymorphic microsatellites from the bovine genome

AU - Ma, R. Z.

AU - Russ, I.

AU - Park, C.

AU - Heyen, D. W.

AU - Beever, J. E.

AU - Green, C. A.

AU - Lewin, H. A.

PY - 1996/2

Y1 - 1996/2

N2 - A small-insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single-stranded pBluescript DNA carrying (CA)n/(TG)n tandem repeats. Approximately 50% of the clones in the enriched library contained (CA)n repeats or CA-rich sequences. Sequencing of clones selected for (CA)n repeats resulted in the identification and characterization of 45 (CA)n polymorphic microsatellites. Genotyping in 9 large paternal half-sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6·3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high-resolution linkage map of bovine genome.

AB - A small-insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single-stranded pBluescript DNA carrying (CA)n/(TG)n tandem repeats. Approximately 50% of the clones in the enriched library contained (CA)n repeats or CA-rich sequences. Sequencing of clones selected for (CA)n repeats resulted in the identification and characterization of 45 (CA)n polymorphic microsatellites. Genotyping in 9 large paternal half-sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6·3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high-resolution linkage map of bovine genome.

KW - Bovine gene mapping

KW - Microsatellites

KW - Polymorphism

UR - https://www.scopus.com/pages/publications/0030088144

UR - https://www.scopus.com/pages/publications/0030088144#tab=citedBy

U2 - 10.1111/j.1365-2052.1996.tb01175.x

DO - 10.1111/j.1365-2052.1996.tb01175.x

M3 - Article

C2 - 8624035

AN - SCOPUS:0030088144

SN - 0268-9146

VL - 27

SP - 43

EP - 47

JO - Animal genetics

JF - Animal genetics

IS - 1

ER -

Isolation and characterization of 45 polymorphic microsatellites from the bovine genome

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this