Isolation and characterization of 45 polymorphic microsatellites from the bovine genome

R. Z. Ma; I. Russ; C. Park; D. W. Heyen; J. E. Beever; C. A. Green; H. A. Lewin

doi:10.1111/j.1365-2052.1996.tb01175.x

Isolation and characterization of 45 polymorphic microsatellites from the bovine genome

R. Z. Ma, I. Russ, C. Park, D. W. Heyen, J. E. Beever, C. A. Green, H. A. Lewin

Animal Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A small-insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single-stranded pBluescript DNA carrying (CA)_n/(TG)_n tandem repeats. Approximately 50% of the clones in the enriched library contained (CA)_n repeats or CA-rich sequences. Sequencing of clones selected for (CA)_n repeats resulted in the identification and characterization of 45 (CA)_n polymorphic microsatellites. Genotyping in 9 large paternal half-sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6·3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high-resolution linkage map of bovine genome.

Original language	English (US)
Pages (from-to)	43-47
Number of pages	5
Journal	Animal genetics
Volume	27
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-2052.1996.tb01175.x
State	Published - Feb 1996

Keywords

Bovine gene mapping
Microsatellites
Polymorphism

ASJC Scopus subject areas

Animal Science and Zoology
Genetics

Online availability

10.1111/j.1365-2052.1996.tb01175.x

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abstract = "A small-insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single-stranded pBluescript DNA carrying (CA)n/(TG)n tandem repeats. Approximately 50% of the clones in the enriched library contained (CA)n repeats or CA-rich sequences. Sequencing of clones selected for (CA)n repeats resulted in the identification and characterization of 45 (CA)n polymorphic microsatellites. Genotyping in 9 large paternal half-sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6·3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high-resolution linkage map of bovine genome.",

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AU - Ma, R. Z.

AU - Russ, I.

AU - Park, C.

AU - Heyen, D. W.

AU - Beever, J. E.

AU - Green, C. A.

AU - Lewin, H. A.

PY - 1996/2

Y1 - 1996/2

N2 - A small-insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single-stranded pBluescript DNA carrying (CA)n/(TG)n tandem repeats. Approximately 50% of the clones in the enriched library contained (CA)n repeats or CA-rich sequences. Sequencing of clones selected for (CA)n repeats resulted in the identification and characterization of 45 (CA)n polymorphic microsatellites. Genotyping in 9 large paternal half-sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6·3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high-resolution linkage map of bovine genome.

AB - A small-insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single-stranded pBluescript DNA carrying (CA)n/(TG)n tandem repeats. Approximately 50% of the clones in the enriched library contained (CA)n repeats or CA-rich sequences. Sequencing of clones selected for (CA)n repeats resulted in the identification and characterization of 45 (CA)n polymorphic microsatellites. Genotyping in 9 large paternal half-sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6·3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high-resolution linkage map of bovine genome.

KW - Bovine gene mapping

KW - Microsatellites

KW - Polymorphism

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Isolation and characterization of 45 polymorphic microsatellites from the bovine genome

Abstract

Keywords

ASJC Scopus subject areas

Online availability

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