Isolation and characterization of 45 polymorphic microsatellites from the bovine genome

R. Z. Ma, I. Russ, C. Park, D. W. Heyen, J. E. Beever, C. A. Green, H. A. Lewin

Research output: Contribution to journalArticlepeer-review

Abstract

A small-insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single-stranded pBluescript DNA carrying (CA)n/(TG)n tandem repeats. Approximately 50% of the clones in the enriched library contained (CA)n repeats or CA-rich sequences. Sequencing of clones selected for (CA)n repeats resulted in the identification and characterization of 45 (CA)n polymorphic microsatellites. Genotyping in 9 large paternal half-sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6·3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high-resolution linkage map of bovine genome.

Original languageEnglish (US)
Pages (from-to)43-47
Number of pages5
JournalAnimal genetics
Volume27
Issue number1
DOIs
StatePublished - Feb 1996

Keywords

  • Bovine gene mapping
  • Microsatellites
  • Polymorphism

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics

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