Is stimulation of leaf photosynthesis by elevated carbon dioxide concentration maintained in the long term? A test with Lolium perenne grown for 10 years at two nitrogen fertilization levels under Free Air CO2 Enrichment (FACE)

Elizabeth Ainsworth, P. A. Davey, G. J. Hymus, C. P. Osborne, A. Rogers, H. Blum, J. Nösberger, Stephen P Long

Research output: Contribution to journalArticle

Abstract

Photosynthesis is commonly stimulated in grasslands with experimental increases in atmospheric CO2 concentration ([CO2]), a physiological response that could significantly alter the future carbon cycle if it persists in the long term. Yet an acclimation of photosynthetic capacity suggested by theoretical models and short-term experiments could completely remove this effect of CO2. Perennial ryegrass (Lolium perenne L. cv. Bastion) was grown under an elevated [CO2] of 600 μmol mol-1 for 10 years using Free Air CO2 Enrichment (FACE), with two contrasting nitrogen levels and abrupt changes in the source: sink ratio following periodic harvests. More than 3000 measurements characterized the response of leaf photosynthesis and stomatal conductance to elevated [CO2] across each growing season for the duration of the experiment. Over the 10 years as a whole, growth at elevated [CO2] resulted in a 43% higher rate of light-saturated leaf photosynthesis and a 36% increase in daily integral of leaf CO2 uptake. Photosynthetic stimulation was maintained despite a 30% decrease in stomatal conductance and significant decreases in both the apparent, maximum carboxylation velocity (Vc,max) and the maximum rate of electron transport (Jmax). Immediately prior to the periodic (every 4-8 weeks) cuts of the L. perenne stands, Vc,max and Jmax, were significantly lower in elevated than in ambient [CO2] in the low-nitrogen treatment. This difference was smaller after the cut, suggesting a dependence upon the balance between the sources and sinks for carbon. In contrast With theoretical expectations and the results of shorter duration experiments, the present results provide no significant change in photosynthetic stimulation across a 10-year period, nor greater acclimation in Vc,max and Jmax in the later years in either nitrogen treatment.

Original languageEnglish (US)
Pages (from-to)705-714
Number of pages10
JournalPlant, Cell and Environment
Volume26
Issue number5
DOIs
StatePublished - May 1 2003

Fingerprint

Lolium
Photosynthesis
Lolium perenne
Fertilization
Carbon Dioxide
Nitrogen
Acclimatization
carbon dioxide
Air
photosynthesis
air
nitrogen
Carbon Sequestration
Carbon Cycle
leaves
testing
Electron Transport
Theoretical Models
Light
stomatal conductance

Keywords

  • Atmospheric change
  • Free Air Carbon dioxide Enrichment
  • Global change
  • Meta-analysis
  • Pasture grass
  • Photosynthesis
  • Rubisco

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

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title = "Is stimulation of leaf photosynthesis by elevated carbon dioxide concentration maintained in the long term? A test with Lolium perenne grown for 10 years at two nitrogen fertilization levels under Free Air CO2 Enrichment (FACE)",
abstract = "Photosynthesis is commonly stimulated in grasslands with experimental increases in atmospheric CO2 concentration ([CO2]), a physiological response that could significantly alter the future carbon cycle if it persists in the long term. Yet an acclimation of photosynthetic capacity suggested by theoretical models and short-term experiments could completely remove this effect of CO2. Perennial ryegrass (Lolium perenne L. cv. Bastion) was grown under an elevated [CO2] of 600 μmol mol-1 for 10 years using Free Air CO2 Enrichment (FACE), with two contrasting nitrogen levels and abrupt changes in the source: sink ratio following periodic harvests. More than 3000 measurements characterized the response of leaf photosynthesis and stomatal conductance to elevated [CO2] across each growing season for the duration of the experiment. Over the 10 years as a whole, growth at elevated [CO2] resulted in a 43{\%} higher rate of light-saturated leaf photosynthesis and a 36{\%} increase in daily integral of leaf CO2 uptake. Photosynthetic stimulation was maintained despite a 30{\%} decrease in stomatal conductance and significant decreases in both the apparent, maximum carboxylation velocity (Vc,max) and the maximum rate of electron transport (Jmax). Immediately prior to the periodic (every 4-8 weeks) cuts of the L. perenne stands, Vc,max and Jmax, were significantly lower in elevated than in ambient [CO2] in the low-nitrogen treatment. This difference was smaller after the cut, suggesting a dependence upon the balance between the sources and sinks for carbon. In contrast With theoretical expectations and the results of shorter duration experiments, the present results provide no significant change in photosynthetic stimulation across a 10-year period, nor greater acclimation in Vc,max and Jmax in the later years in either nitrogen treatment.",
keywords = "Atmospheric change, Free Air Carbon dioxide Enrichment, Global change, Meta-analysis, Pasture grass, Photosynthesis, Rubisco",
author = "Elizabeth Ainsworth and Davey, {P. A.} and Hymus, {G. J.} and Osborne, {C. P.} and A. Rogers and H. Blum and J. N{\"o}sberger and Long, {Stephen P}",
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T1 - Is stimulation of leaf photosynthesis by elevated carbon dioxide concentration maintained in the long term? A test with Lolium perenne grown for 10 years at two nitrogen fertilization levels under Free Air CO2 Enrichment (FACE)

AU - Ainsworth, Elizabeth

AU - Davey, P. A.

AU - Hymus, G. J.

AU - Osborne, C. P.

AU - Rogers, A.

AU - Blum, H.

AU - Nösberger, J.

AU - Long, Stephen P

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Photosynthesis is commonly stimulated in grasslands with experimental increases in atmospheric CO2 concentration ([CO2]), a physiological response that could significantly alter the future carbon cycle if it persists in the long term. Yet an acclimation of photosynthetic capacity suggested by theoretical models and short-term experiments could completely remove this effect of CO2. Perennial ryegrass (Lolium perenne L. cv. Bastion) was grown under an elevated [CO2] of 600 μmol mol-1 for 10 years using Free Air CO2 Enrichment (FACE), with two contrasting nitrogen levels and abrupt changes in the source: sink ratio following periodic harvests. More than 3000 measurements characterized the response of leaf photosynthesis and stomatal conductance to elevated [CO2] across each growing season for the duration of the experiment. Over the 10 years as a whole, growth at elevated [CO2] resulted in a 43% higher rate of light-saturated leaf photosynthesis and a 36% increase in daily integral of leaf CO2 uptake. Photosynthetic stimulation was maintained despite a 30% decrease in stomatal conductance and significant decreases in both the apparent, maximum carboxylation velocity (Vc,max) and the maximum rate of electron transport (Jmax). Immediately prior to the periodic (every 4-8 weeks) cuts of the L. perenne stands, Vc,max and Jmax, were significantly lower in elevated than in ambient [CO2] in the low-nitrogen treatment. This difference was smaller after the cut, suggesting a dependence upon the balance between the sources and sinks for carbon. In contrast With theoretical expectations and the results of shorter duration experiments, the present results provide no significant change in photosynthetic stimulation across a 10-year period, nor greater acclimation in Vc,max and Jmax in the later years in either nitrogen treatment.

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KW - Atmospheric change

KW - Free Air Carbon dioxide Enrichment

KW - Global change

KW - Meta-analysis

KW - Pasture grass

KW - Photosynthesis

KW - Rubisco

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