Investigation of developing embryonic morphology using optical coherence tomography

Stephen A. Boppart, Mark E. Brezinski, Brett E. Bouma, Guillermo J. Tearney, James G. Fujimoto

Research output: Contribution to journalArticlepeer-review


Improved imaging of morphological changes has the potential of offering new insight into the complex process of embryonic development. Optical coherence tomography (OCT) is a new imaging technique for performing in vivo cross-sectional imaging of architectural morphology by measuring backscattered infrared light. This study investigates the application of OCT for imaging developing structure in Rana pipiens, Xenopus laevis, and Brachydanio rerio. Images are compared to conventional histological baselines. Cross-sectional imaging can be performed and structural morphology identified at greater imaging depths than possible with confocal and light microscopy. Repeated OCT imaging may be performed in vivo in order to track structural changes throughout development.

Original languageEnglish (US)
Pages (from-to)54-63
Number of pages10
JournalDevelopmental Biology
Issue number1
StatePublished - Jul 10 1996
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


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