Investigation of 3D microscopy using intensity diffraction tomography

Mark A. Anastasio, Yin Huang, Greg Gbur, Paul Scott Carney

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Diffraction tomography (DT) is an established imaging technique for reconstructing the complex-valued refractive index distribution of a weakly scattering 3D sample. Due to experimental difficulties associated with the direct measurement of the phase of an optical wavefield, the effectiveness of DT for optical imaging applications has been limited. A theory of intensity diffraction tomography (I-DT) has been proposed to circumvent this phase retrieval problem. In this work, we review the features of I-DT reconstruction theory that are relevant to optical microscopy. Computer-simulation studies are conducted to investigate the performance of reconstruction algorithms for a proposed I-DT microscope. The effects of data noise are assessed, and statistically optimal reconstruction strategies that employ multiple detector planes are proposed.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
DOIs
StatePublished - May 8 2006
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII - San Jose, CA, United States
Duration: Jan 24 2006Jan 26 2006

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6090
ISSN (Print)0277-786X

Other

OtherThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII
CountryUnited States
CitySan Jose, CA
Period1/24/061/26/06

Keywords

  • Diffraction tomography
  • Three-dimensional microscopy
  • Tomographic image reconstruction

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Anastasio, M. A., Huang, Y., Gbur, G., & Carney, P. S. (2006). Investigation of 3D microscopy using intensity diffraction tomography. In Proceedings of SPIE - The International Society for Optical Engineering [60900G] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6090). https://doi.org/10.1117/12.648169