@inproceedings{37eaf184090b4b0fb2ebcb105fb042de,
title = "Investigating the Link between Fermentative Metabolism and Hydrogen Production in the Unicellular Green Alga Chlamydomonas Reinhardtii",
abstract = "In the model green alga Chlamydomonas reinhardtii, the electrons required for hydrogen production can come from both the biophotolysis of water and from the fermentation of carbohydrate reserves. Anoxia leads to the activation of several fermentative pathways, which produce a number of end products including formic, malic and acetic acid along with ethanol, carbon dioxide and hydrogen. It has been proposed that by switching off competing fermentative pathways hydrogen production can be increased. Therefore the aim of this study was to devise an experimental strategy to down-regulate the expression of enzymes thought to control C. reinhardtii's fermentative metabolism. We demonstrate here that it is possible to use artificial microRNA (amiRNA) technology to generate knock-down mutants with reduced expression of pyruvate formate lyase (PFL1), a key fermentative enzyme in C. reinhardtii. This work opens up new possibilities to improve hydrogen yields through metabolic engineering.",
author = "Burgess, {S. J.} and Nixon, {P. J.}",
note = "Publisher Copyright: {\textcopyright} 2010 18th World Hydrogen Energy Conference 2010, WHEC 2010, Proceedings. All Rights Reserved.; 18th World Hydrogen Energy Conference, WHEC 2010 ; Conference date: 16-05-2010 Through 21-05-2010",
year = "2010",
language = "English (US)",
series = "18th World Hydrogen Energy Conference 2010, WHEC 2010, Proceedings",
publisher = "Forschungszentrum Julich GmbH",
pages = "111--116",
editor = "Detlef Stolten and Thomas Grube",
booktitle = "Hydrogen Production Technologies - Part 1",
}