Investigating brain d-serine: Advocacy for good practices

Jean Pierre Mothet, Jean Marie Billard, Loredano Pollegioni, Joseph T. Coyle, Jonathan V Sweedler

Research output: Contribution to journalReview article

Abstract

The last two decades have witnessed remarkable advance in our understanding the role of d-amino acids in the mammalian nervous system: from the unknown, to known molecules with unknown functions, to potential central players in health and disease. d-Amino acids have emerged as an important class of signaling molecules. In particular, the exploration of the roles of d-serine in brain physiopathology is a vibrant field that is growing at an accelerating pace. However, disentangling the functions of a chiral molecule in a complex chemical matrice as the brain requires specific measurement and detection methods but is also a challenging task as many molecular tools and models investigators are using can lead to confounded observations. Thus, study of d-amino acids demands accurate methodologies and specific controls, and these have often been lacking. Here we outline best practices for d-amino acid research, with a special emphasis on d-serine. We hope these concepts help move the field to greater rigor and reproducibility, allowing the field to advance.

Original languageEnglish (US)
Article numbere13257
JournalActa Physiologica
Volume226
Issue number1
DOIs
StatePublished - May 2019

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Serine
Amino Acids
Brain
Molecular Models
Practice Guidelines
Nervous System
Research Personnel
Health
Research

Keywords

  • NMDA receptors
  • analytical methods
  • d-Serine
  • glia
  • immunostainings
  • neurons
  • rescue experiments
  • serine racemase inhibitors

ASJC Scopus subject areas

  • Physiology

Cite this

Mothet, J. P., Billard, J. M., Pollegioni, L., Coyle, J. T., & Sweedler, J. V. (2019). Investigating brain d-serine: Advocacy for good practices. Acta Physiologica, 226(1), [e13257]. https://doi.org/10.1111/apha.13257

Investigating brain d-serine : Advocacy for good practices. / Mothet, Jean Pierre; Billard, Jean Marie; Pollegioni, Loredano; Coyle, Joseph T.; Sweedler, Jonathan V.

In: Acta Physiologica, Vol. 226, No. 1, e13257, 05.2019.

Research output: Contribution to journalReview article

Mothet, JP, Billard, JM, Pollegioni, L, Coyle, JT & Sweedler, JV 2019, 'Investigating brain d-serine: Advocacy for good practices', Acta Physiologica, vol. 226, no. 1, e13257. https://doi.org/10.1111/apha.13257
Mothet, Jean Pierre ; Billard, Jean Marie ; Pollegioni, Loredano ; Coyle, Joseph T. ; Sweedler, Jonathan V. / Investigating brain d-serine : Advocacy for good practices. In: Acta Physiologica. 2019 ; Vol. 226, No. 1.
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