Investigating brain d-serine: Advocacy for good practices

Jean Pierre Mothet, Jean Marie Billard, Loredano Pollegioni, Joseph T. Coyle, Jonathan V. Sweedler

Research output: Contribution to journalReview articlepeer-review


The last two decades have witnessed remarkable advance in our understanding the role of d-amino acids in the mammalian nervous system: from the unknown, to known molecules with unknown functions, to potential central players in health and disease. d-Amino acids have emerged as an important class of signaling molecules. In particular, the exploration of the roles of d-serine in brain physiopathology is a vibrant field that is growing at an accelerating pace. However, disentangling the functions of a chiral molecule in a complex chemical matrice as the brain requires specific measurement and detection methods but is also a challenging task as many molecular tools and models investigators are using can lead to confounded observations. Thus, study of d-amino acids demands accurate methodologies and specific controls, and these have often been lacking. Here we outline best practices for d-amino acid research, with a special emphasis on d-serine. We hope these concepts help move the field to greater rigor and reproducibility, allowing the field to advance.

Original languageEnglish (US)
Article numbere13257
JournalActa Physiologica
Issue number1
StatePublished - May 2019


  • NMDA receptors
  • analytical methods
  • d-Serine
  • glia
  • immunostainings
  • neurons
  • rescue experiments
  • serine racemase inhibitors

ASJC Scopus subject areas

  • Physiology


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