Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

Weng Lin Tang, Zhi Li, Huimin Zhao

Research output: Contribution to journalArticle

Abstract

We report the first example of directed evolution of a P450 monooxygenase with inverted enantioselectivity for asymmetric biohydroxylation. The biohydroxylation product of the best mutant 1AF4A has an ee of 83% (R) compared to the wild type's ee of 43% (S).

Original languageEnglish (US)
Pages (from-to)5461-5463
Number of pages3
JournalChemical Communications
Volume46
Issue number30
DOIs
StatePublished - Aug 14 2010

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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