Intraplant nuclear DNA content variation in diploid nuclei of maize (Zea mays L.)

D. P. Biradar, A Lane Rayburn

Research output: Contribution to journalArticle

Abstract

Diploid nuclei from stem, mesocotyl, nodal root and root tip tissue of two maize hybrids were examined with respect to their DNA content. The nuclei were isolated and stained with DAPI and passed through a flow cytometer-cell sorter. The titration curve for each tissue was determined. Significant variation was observed among nuclei of different tissue types. Stem and root tips had the highest diploid nuclear DNA amounts while 2-week-old mesocotyl had the lowest diploid nuclear DNA amount. These results provide evidence that during plant development and differentiation, the amount of DNA within a diploid nucleus changes through loss of specific DNA sequences. This study also demonstrates the sensitivity of flow cytometry in detecting small intraplant variation in nuclear DNA.

Original languageEnglish (US)
Pages (from-to)1039-1044
Number of pages6
JournalJournal of experimental botany
Volume44
Issue number6
DOIs
StatePublished - Jun 1 1993

Fingerprint

Zea Mays L.
Maize
Diploidy
nuclear genome
Nucleus
Zea mays
diploidy
DNA
maize
corn
Roots
root tips
Meristem
Tissue
Flow Cytometry
stems
titration
stem
DNA Sequence
Plant Development

Keywords

  • DNA content
  • Flow cytometry
  • Fluorochrome DAPI
  • Plant development
  • Tissue differentiation

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Intraplant nuclear DNA content variation in diploid nuclei of maize (Zea mays L.). / Biradar, D. P.; Rayburn, A Lane.

In: Journal of experimental botany, Vol. 44, No. 6, 01.06.1993, p. 1039-1044.

Research output: Contribution to journalArticle

@article{1891c20d54d04806985550419ac2c1ed,
title = "Intraplant nuclear DNA content variation in diploid nuclei of maize (Zea mays L.)",
abstract = "Diploid nuclei from stem, mesocotyl, nodal root and root tip tissue of two maize hybrids were examined with respect to their DNA content. The nuclei were isolated and stained with DAPI and passed through a flow cytometer-cell sorter. The titration curve for each tissue was determined. Significant variation was observed among nuclei of different tissue types. Stem and root tips had the highest diploid nuclear DNA amounts while 2-week-old mesocotyl had the lowest diploid nuclear DNA amount. These results provide evidence that during plant development and differentiation, the amount of DNA within a diploid nucleus changes through loss of specific DNA sequences. This study also demonstrates the sensitivity of flow cytometry in detecting small intraplant variation in nuclear DNA.",
keywords = "DNA content, Flow cytometry, Fluorochrome DAPI, Plant development, Tissue differentiation",
author = "Biradar, {D. P.} and Rayburn, {A Lane}",
year = "1993",
month = "6",
day = "1",
doi = "10.1093/jxb/44.6.1039",
language = "English (US)",
volume = "44",
pages = "1039--1044",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Intraplant nuclear DNA content variation in diploid nuclei of maize (Zea mays L.)

AU - Biradar, D. P.

AU - Rayburn, A Lane

PY - 1993/6/1

Y1 - 1993/6/1

N2 - Diploid nuclei from stem, mesocotyl, nodal root and root tip tissue of two maize hybrids were examined with respect to their DNA content. The nuclei were isolated and stained with DAPI and passed through a flow cytometer-cell sorter. The titration curve for each tissue was determined. Significant variation was observed among nuclei of different tissue types. Stem and root tips had the highest diploid nuclear DNA amounts while 2-week-old mesocotyl had the lowest diploid nuclear DNA amount. These results provide evidence that during plant development and differentiation, the amount of DNA within a diploid nucleus changes through loss of specific DNA sequences. This study also demonstrates the sensitivity of flow cytometry in detecting small intraplant variation in nuclear DNA.

AB - Diploid nuclei from stem, mesocotyl, nodal root and root tip tissue of two maize hybrids were examined with respect to their DNA content. The nuclei were isolated and stained with DAPI and passed through a flow cytometer-cell sorter. The titration curve for each tissue was determined. Significant variation was observed among nuclei of different tissue types. Stem and root tips had the highest diploid nuclear DNA amounts while 2-week-old mesocotyl had the lowest diploid nuclear DNA amount. These results provide evidence that during plant development and differentiation, the amount of DNA within a diploid nucleus changes through loss of specific DNA sequences. This study also demonstrates the sensitivity of flow cytometry in detecting small intraplant variation in nuclear DNA.

KW - DNA content

KW - Flow cytometry

KW - Fluorochrome DAPI

KW - Plant development

KW - Tissue differentiation

UR - http://www.scopus.com/inward/record.url?scp=0000353457&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000353457&partnerID=8YFLogxK

U2 - 10.1093/jxb/44.6.1039

DO - 10.1093/jxb/44.6.1039

M3 - Article

AN - SCOPUS:0000353457

VL - 44

SP - 1039

EP - 1044

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 6

ER -