Intracytoplasmic inclusions in circulating leukocytes from an eastern box turtle (Terrapene carolina carolina) with iridoviral infection

Matthew C. Allender, Michael M. Fry, Armando R. Irizarry, Linden Craig, April J. Johnson, Michael Jones

Research output: Contribution to journalArticle

Abstract

A free-ranging adult female eastern box turtle (Terrapene carolina carolina) was presented to the University of Tennessee in October 2003 because of suspected trauma and blindness. Physical examination revealed lethargy, clear ocular and nasal discharges, and white oral and laryngeal plaques. Intracytoplasmic inclusions within heterophils and large mononuclear leukocytes were observed on routine blood smear examination. Postmortem findings included necrosis of epithelial and parenchymal cells with intracytoplasmic inclusions. Ultrastructurally, the leukocyte inclusions consisted of variably electron-dense granular material and viral particles consistent with the Iridoviridae family of viruses. The virus shared 100% sequence identity to a 420-base pair sequence of frog virus 3 (family Iridoviridae, genus Ranavirus) as determined by polymerase chain reaction and gene sequencing targeting a portion of the Ranavirus major capsid protein gene.

Original languageEnglish (US)
Pages (from-to)677-684
Number of pages8
JournalJournal of wildlife diseases
Volume42
Issue number3
DOIs
StatePublished - Jul 2006

Keywords

  • Electron microscopy
  • Frog virus 3
  • Intracytoplasmic inclusion
  • Iridovirus
  • Leukocyte
  • PCR
  • Ranavirus
  • Terrapene carolina

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Fingerprint Dive into the research topics of 'Intracytoplasmic inclusions in circulating leukocytes from an eastern box turtle (Terrapene carolina carolina) with iridoviral infection'. Together they form a unique fingerprint.

  • Cite this